Reference Report

Reference
Citation	Banerjee, S., Lee, J., Venkatesh, K., Wu, C.F., Hasan, G. (2004). Loss of flight and associated neuronal rhythmicity in inositol 1,4,5-trisphosphate receptor mutants of Drosophila. J. Neurosci. 24(36): 7869--7878. (Export to RIS)
FlyBase ID	FBrf0180425
Publication Type	Research paper
PubMed ID	15356199
PubMed Abstract	Coordinated flight in winged insects requires rhythmic activity of the underlying neural circuit. Here, we show that Drosophila mutants for the inositol 1,4,5-trisphosphate (InsP(3)) receptor gene (itpr) are flightless. Electrophysiological recordings from thoracic indirect flight muscles show increased spontaneous firing accompanied by a loss of rhythmic flight activity patterns normally generated in response to a gentle puff of air. In contrast, climbing speed, the jump response, and electrical properties of the giant fiber pathway are normal, indicating that general motor coordination and neuronal excitability are much less sensitive to itpr mutations. All mutant phenotypes are rescued by expression of an itpr(+) transgene in serotonin and dopamine neurons. Pharmacological and immunohistochemical experiments support the idea that the InsP(3) receptor functions to modulate flight specifically through serotonergic interneurons. InsP(3) receptor action appears to be important for normal development of the flight circuit and its central pattern generator.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Alleles (16)
	Avic\GFP^Scer\UAS.cUa Ctet\TeTxLC^Scer\UAS.cKa Itp-r83A^ka1091 Itp-r83A^sa54 Itp-r83A^Scer\UAS.cVa Itp-r83A^ug3 Itp-r83A^wc361 Itp-r83A^wc703 Scer\GAL4^Cha.7.4 Scer\GAL4^Ddc.PL Scer\GAL4^elav.PLu Scer\GAL4^hs.PB Scer\GAL4^Mef2.PR Scer\GAL4^{neur-GAL4-A101} Scer\GAL4^Rapgap1-OK6 Scer\GAL4^{shakB.lethal.4.1}
Constructs (9)
	P{Cha-GAL4.7.4} P{Ddc-GAL4.L} P{GAL4-elav.L} P{GAL4-Hsp70.PB} P{GAL4-Mef2.R} P{shakB(lethal)-GAL4.4.1} P{UAS(-FRT)TeTxLC} P{UAS-GFP.U} P{UAS-Itp-r83A.V}
Genes (4)
	Avic\GFP Ctet\TeTxLC Itp-r83A Scer\GAL4
Insertions (2)
	P{GawB}neur^GAL4-A101 P{GawB}OK6