Reference Report

Reference
Citation	Vereshchagina, N., Bennett, D., Szoor, B., Kirchner, J., Gross, S., Vissi, E., White-Cooper, H., Alphey, L. (2004). the essential role of PP1beta in Drosophila is to regulate nonmuscle myosin. Mol. Biol. Cell 15(10): 4395--4405. (Export to RIS)
FlyBase ID	FBrf0180501
Publication Type	Research paper
PubMed ID	15269282
PubMed Abstract	Reversible phosphorylation of myosin regulatory light chain (MRLC) is a key regulatory mechanism controlling myosin activity and thus regulating the actin/myosin cytoskeleton. We show that Drosophila PP1beta, a specific isoform of serine/threonine protein phosphatase 1 (PP1), regulates nonmuscle myosin and that this is the essential role of PP1beta. Loss of PP1beta leads to increased levels of phosphorylated nonmuscle MRLC (Sqh) and actin disorganisation; these phenotypes can be suppressed by reducing the amount of active myosin. Drosophila has two nonmuscle myosin targeting subunits, one of which (MYPT-75D) resembles MYPT3, binds specifically to PP1beta, and activates PP1beta's Sqh phosphatase activity. Expression of a mutant form of MYPT-75D that is unable to bind PP1 results in elevation of Sqh phosphorylation in vivo and leads to phenotypes that can also be suppressed by reducing the amount of active myosin. The similarity between fly and human PP1beta and MYPT genes suggests this role may be conserved.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Alleles (34)
	Avic\GFP^Ubi-p63E.PD flw¹ flw⁶ flw^G0172 flw^Scer\UAS.cRa flw^{Scer\UAS.T:Ivir\HA1} Mbs³ Mbs^L.hs MYPT-75D^{F117A.Scer\UAS} MYPT-75D^HM.Scer\UAS Pp1-87B¹ Pp1-87B^87Bg-3 Pp1-87B^{Scer\UAS.T:Ivir\HA1} Rho1^E3.10 RhoGEF2⁰⁴²⁹¹ Scer\GAL4^arm.PS Scer\GAL4^Bx-MS1096 Scer\GAL4^hs.PB sqh^{A20.A21.T:Zzzz\FLAG} sqh^{A20.T:Zzzz\FLAG} sqh^{A21.T:Zzzz\FLAG} sqh^E20.E21 sqh^{E21.T:Zzzz\FLAG} sqh^T:Zzzz\FLAG Tm1⁰²²⁹⁹ Tm1^Su(flw)4 Tm2^J8 w^+mC zip¹ zip⁰²⁹⁵⁷ zip^IIX62 zip^Su(flw)1 zip^Su(flw)2 zip^Su(flw)3
Constructs (15)
	P{GAL4-arm.S} P{GAL4-Hsp70.PB} P{hs-Mbs.L} P{sqh-A20,A21} P{sqh-A20} P{sqh-A21} P{sqh-E20E21} P{sqh-E21} P{sqh-FLAG} P{UAS-flw.HA} P{UAS-MYPT-75D.F117A} P{UAS-MYPT-75D.HM} P{UAS-Pp1-87B.HA} P{UAS-Pp1β9C} P{Ubi-GFP.D}
Genes (18)
	Act5C Act42A Avic\GFP flw Mbs MYPT-75D Pp1-87B Rho1 RhoGEF2 Scer\GAL4 sqh Strn-Mlck T:Ivir\HA1 T:Zzzz\FLAG Tm1 Tm2 w zip
Insertions (4)
	P{GawB}Bx^MS1096 P{PZ}RhoGEF2⁰⁴²⁹¹ P{PZ}Tm1⁰²²⁹⁹ P{PZ}zip⁰²⁹⁵⁷