Reference Report

Reference
Citation	Huang, F.D., Matthies, H.J., Speese, S.D., Smith, M.A., Broadie, K. (2004). Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction. Nat. Neurosci. 7(10): 1070--1078. (Export to RIS)
FlyBase ID	FBrf0180564
Publication Type	Research paper
PubMed ID	15361878
PubMed Abstract	The rolling blackout (rbo) gene encodes an integral plasma membrane lipase required for Drosophila phototransduction. Photoreceptors are enriched for the RBO protein, and temperature-sensitive rbo mutants show reversible elimination of phototransduction within minutes, demonstrating an acute requirement for the protein. The block is activity dependent, indicating that the action of RBO is use dependent. Conditional rbo mutants show activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate and phosphatidylinositol 4,5-bisphosphate within minutes of induction, suggesting rapid downregulation of phospholipase C (PLC) activity. The RBO requirement identifies an essential regulatory step in G-protein-coupled, PLC-dependent inositol lipid signaling mediating activation of TRP and TRPL channels during phototransduction.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Neurosci.
Title	Nature Neuroscience
Publication Year	1998-
ISBN/ISSN	1097-6256
Data from Reference
Aberrations (6)
	Df(2R)cn88b Df(2R)H3C1 Df(2R)H3D3 Df(2R)nap1 Df(2R)nap9 Df(2R)Np3
Alleles (12)
	stmA¹ stmA^18-2 stmA^cmp44E-1 stmA^{MtnA.T:SV5\V5} stmA^rbo-P1 stmA^rbo-P2 stmA^rev499 stmA^{T:Avic\GFP-EGFP} T:Avic\GFP^EGFP trp⁹ trpl³⁰² w^+mC
Constructs (2)
	P{lacW} P{stmA-EGFP.H}
Genes (5)
	stmA T:SV5\V5 trp trpl w
Insertions (2)
	P{lacW}stmA^rbo-P1 P{lacW}stmA^rbo-P2
Natural transposons (1)
	P-element