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Reference Report
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| Citation | Bellen, H. (2005.1.25). spdo/tmod split. (Export to RIS) | ||
| FlyBase ID | FBrf0182569 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
Date: Fri, 21 Jan 2005 09:44:23 \-0600
To: David Sutherland <djs93XXXXXXXXXXXXXXX>, jskeathXXXXXXXXXXXXXXX From: Hugo Bellen <hbellen@XXXXXXXXXXXXXXX> Subject: Re: spdo/tmod split Hi, At 02:26 PM 1/21/2005 \+0000, you wrote: >Dear Hugo and James, >>I'm currently engaged in the tricky task of trying to split FlyBase's current >record for spdo into 2 (tmod/CG1539 & spdo/CG31020), based on the data in >'O'Connor-Giles and Skeath, 2003, Dev. Cell 5(2): 231--243'. >>I'd like to check with you that my proposed partitioning of the alleles fits >with your understanding of their behaviour. Any additional >complementation or >molecular data that could help with this partitioning would be most >welcome. I >can curate such data as a personal communication to FlyBase. >>The tough question is what to do with 'spdoH7'(*) and its associated data. >> On the basis of the mapped insertion 'P{lacW}spdoH7' (now to be renamed >P{lacW}tmod)H7) it needs to be an allele of tmod. On the basis of >failure to >complement spdoC55 (see below), it needs to be an allele of spdo. The most >obvious explanation is that the 'H7' chromosome has mutations in both genes \- >suggesting I should make both alleles. This still leaves the problem of >where >to put the phenotypic data. It most likely hits two or more genes. As far as I can establish, we do not know what the phenotype is of tmod, but Jim can prove me wrong. >Any suggestions? >>Here is how I plan to split the rest of the alleles between the two genes: >>Alleles remaining spdo/CG31020: >>1. leisions mapped by O'Conner-Giles & Skeath: >>spdoC55>spdoG104>spdoK433>>spdoZZ27 (a deficiency removing spdo/CG31020 : data from FBrf0162056 == >O'Connor-Giles and Skeath, 2003, Dev. Cell) > Strictly we only make alleles from Dfs when one of their breakpoints is > in the >gene in question \- Is this the case? spdoZZ27 is a deletion that removes both tmod and spdo, and other genes. It is a null for tmod (no transcript) >2. On the basis of complementation: >>spdoS097002b>Separable from: P{lacW}S097002a >data from: FBrf0099763 Salzberg et al., 1997: >Fails to complement: spdoC55>Southern analysis reveals a chromosomal rearrangement. >Lethality not revertible by Δ2-3-induced mobilization >>Fails to complement: spdoC55>Isolation of spdoH7 by failure to complement spdoC55 is described in >'Dye et al., '98 FBrf0102830'>>Alleles to split out as tmod: >1. constructs: >spdohs.PDL is the only one of these >'rescue' data for 'spdoH7' \- should this now be recorded as partial >suppression of a spdoH7 phenotype by hs-tmod? That is the best explanation as the partial rescue in the PNS was repeated blind. >2. Insertions mapped to CG1539: >>spdo02288>spdo00848>>3. lesions mapped to CG1539 >>spdoS130910>A portion of the CG1539 gene is deleted. (data Dye et al., '98 FBrf0102830) Correct>spdoZZ27 (a deficiency removing tmod/CG1539: data from FBrf0162056 == >O'Connor-Giles and Skeath, 2003, Dev. Cell) > Strictly we only make alleles from Dfs when one of their breakpoints is > in the >gene in question \- Is this the case? Jim may know the precise breakpoint..>>4. On basis of complementation only: >>spdorG347>Fails to complement: spdo00848>(data from BDGP) >>Thanks in advance Sorry for the confusion, but double hits with P-elements, the deletion associated with zz27, and the partial rescue, are at the base of much of the problems. Please confer with Jim, but I think you got most of it... Hugo |
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| Language of Publication | English | ||
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Data from Reference
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| Aberrations (1)
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| Alleles (11)
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| Genes (2)
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