Reference Report

Reference
Citation	Kuzin, A., Brody, T., Moore, A.W., Odenwald, W.F. (2005). Nerfin-1 is required for early axon guidance decisions in the developing Drosophila CNS. Dev. Biol. 277(2): 347--365. (Export to RIS)
FlyBase ID	FBrf0183075
Publication Type	Research paper
PubMed ID	15617679
PubMed Abstract	Many studies have focused on the mechanisms of axon guidance; however, little is known about the transcriptional control of the navigational components that carryout these decisions. This report describes the functional analysis of Nerfin-1, a nuclear regulator of axon guidance required for a subset of early pathfinding events in the developing Drosophila CNS. Nerfin-1 belongs to a highly conserved subfamily of Zn-finger proteins with cognates identified in nematodes and man. We show that the neural precursor gene prospero is essential for nerfin-1 expression. Unlike nerfin-1 mRNA, which is expressed in many neural precursor cells, the encoded Nerfin-1 protein is only detected in the nuclei of neuronal precursors that will divide just once and then transiently in their nascent neurons. Although nerfin-1 null embryos have no discernible alterations in neural lineage development nor in neuronal or glial identities, CNS pioneering neurons require nerfin-1 function for early axon guidance decisions. Furthermore, nerfin-1 is required for the proper development of commissural and connective axon fascicles. Our studies also show that Nerfin-1 is essential for the proper expression of robo2, wnt5, derailed, G-oalpha47A, Lar, and futsch, genes whose encoded proteins participate in these early navigational events.
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Supplementary material	Supplemental materials. [FBrf0183076]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (3)
	Df(3L)nerfin-1^1B Df(3L)nerfin-1^2B Df(3L)nerfin-1^CM23
Alleles (11)
	Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} nerfin-1⁵⁴ nerfin-1¹⁵⁹ nerfin-1^+t11.154 nerfin-1^Scer\UAS.cKa pros^Il3 Scer\GAL4^cas-3921 Scer\GAL4^elav-C155 Scer\GAL4^pros.PMG Scer\GAL4^sca-537.4 Scer\GAL4^twi.PB
Constructs (5)
	P{GAL4-pros.MG} P{GAL4-twi.B} P{nerfin-1^+t11.154} P{UAS-mCD8::GFP.L} P{UAS-nerfin-1}
Genes (65)
	acj6 bif CadN caps Cdk5 CG13920 CG16778 comm Con cpo ct danr dock drl elav en Eph Ephrin eve Fas1 Fas2 fra fru futsch Gαo Gαs hb HGTX hiw Kr Lar lbm lea lin-28 lola Mmus\Cd8a nerfin-1 NetA NetB Nrg Nrt nub odd Oli Pak pod1 pros Ptp10D Ptp69D Ptp99A repo rhea robo robo3 Scer\GAL4 Sema-1a sli sm Syx1A T:Avic\GFP tap trio unc-5 Wnt5 wrapper
Insertions (3)
	P{GAL4}cas³⁹²¹ P{GawB}elav^C155 P{GawB}sca^537.4