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Citation
Mok, L.P., Qin, T., Bardot, B., LeComte, M., Homayouni, A., Ahimou, F., Wesley, C. (2005). Delta activity independent of its activity as a ligand of Notch.  BMC Dev. Biol. 5(1): 6.
FlyBase ID
FBrf0183736
Publication Type
Research paper
Abstract

Delta, Notch, and Scabrous often function together to make different cell types and refine tissue patterns during Drosophila development. Delta is known as the ligand that triggers Notch receptor activity. Scabrous is known to bind Notch and promote Notch activity in response to Delta. It is not known if Scabrous binds Delta or Delta has activity other than its activity as a ligand of Notch. It is very difficult to clearly determine this binding or activity in vivo as all Notch, Delta, and Scabrous activities are required simultaneously or successively in an inter-dependent manner.Using Drosophila cultured cells we show that the full length Delta promotes accumulation of Daughterless protein, fringe RNA, and pangolin RNA in the absence of Scabrous or Notch. Scabrous binds Delta and suppresses this activity even though it increases the level of the Delta intracellular domain. We also show that Scabrous can promote Notch receptor activity, in the absence of Delta.Delta has activity that is independent of its activity as a ligand of Notch. Scabrous suppresses this Delta activity. Scabrous also promotes Notch activity that is dependent on Delta's ligand activity. Thus, Notch, Delta, and Scabrous might function in complex combinatorial or mutually exclusive interactions during development. The data reported here will be of significant help in understanding these interactions in vivo.

PubMed ID
PubMed Central ID
PMC555533 (PMC) (EuropePMC)
Associated Information
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Secondary IDs
  • FBrf0189760
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
BMC Dev. Biol.
Title
BMC Developmental Biology
Publication Year
2002
ISBN/ISSN
1471-213X
Data From Reference
Genes (25)
Physical Interactions (3)
Cell Lines (3)