Reference Report

Reference
Citation	Stumpff, J., Duncan, T., Homola, E., Campbell, S.D., Su, T.T. (2004). Drosophila Wee1 kinase regulates Cdk1 and mitotic entry during embryogenesis. Curr. Biol. 14(23): 2143--2148. (Export to RIS)
FlyBase ID	FBrf0183813
Publication Type	Research paper
PubMed ID	15589158
PubMed Abstract	Cyclin-dependent kinases (Cdks) are the central regulators of the cell division cycle. Inhibitors of Cdks ensure proper coordination of cell cycle events and help regulate cell proliferation in the context of tissues and organs. Wee1 homologs phosphorylate a conserved tyrosine to inhibit the mitotic cyclin-dependent kinase Cdk1. Loss of Wee1 function in fission or budding yeast causes premature entry into mitosis. The importance of metazoan Wee1 homologs for timing mitosis, however, has been demonstrated only in Xenopus egg extracts and via ectopic Cdk1 activation . Here, we report that Drosophila Wee1 (dWee1) regulates Cdk1 via phosphorylation of tyrosine 15 and times mitotic entry during the cortical nuclear cycles of syncytial blastoderm embryos, which lack gap phases. Loss of maternal dwee1 leads to premature entry into mitosis, mitotic spindle defects, chromosome condensation problems, and a Chk2-dependent block of subsequent development, and then embryonic lethality. These findings modify previous models about cell cycle regulation in syncytial embryos and demonstrate that Wee1 kinases can regulate mitotic entry in vivo during metazoan development even in cycles that lack a G2 phase.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (1)
	Df(2L)Dwee1-W05
Alleles (6)
	Avic\GFP^{Jupiter-G00147} His2Av^{T:Avic\GFP-S65T} Jupiter^G00147 lok^p6 T:Avic\GFP^S65T wee^ES1
Constructs (1)
	P{His2Av^{T:Avic\GFP-S65T}}
Genes (13)
	Avic\GFP cdc2 CycB Grip84 His2Av Jupiter lok wee αTub67C αTub84B αTub84D γTub23C γTub37C
Insertions (1)
	P{PTT-GA}Jupiter^G00147