Reference Report

Reference
Citation	Campbell, G. (2005). Regulation of gene expression in the distal region of the Drosophila leg by the Hox11 homolog, C15. Dev. Biol. 278(2): 607--618. (Export to RIS)
FlyBase ID	FBrf0183856
Publication Type	Research paper
PubMed ID	15680373
PubMed Abstract	The distal region of the Drosophila leg, the tarsus, is divided into five segments (ta I-V) and terminates in the pretarsus, which is characterized by a pair of claws. Several homeobox genes are expressed in distinct regions of the tarsus, including aristaless (al) and lim1 in the pretarsus, Bar (B) in ta IV and V, and apterous (ap) in ta IV. This pattern is governed by regulatory interactions between these genes; for example, Al and B are mutually antagonistic resulting in exclusion of B expression from the pretarsus. Although Al is necessary, it is not sufficient to repress B, indicating another factor is required. Here, this factor is identified as the product of the C15 gene, which is another homeodomain protein, a homolog of the human Hox11 oncogene. C15 is expressed in the same cells as al and, together, C15 and Al appear to directly repress B. C15/Al also act indirectly to repress ap in ta V, i.e., in surrounding cells. To do this, C15/Al autonomously repress expression of the gene encoding the Notch ligand Delta (Dl) in the pretarsus, restricting Dl to ta V and creating a Dl+/Dl- border at the interface between ta V and the pretarsus. This results in upregulation of Notch signaling, which induces expression of the bowl gene, the product of which represses ap.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (4)
	Ab(2;?)al^ice Df(3R)e-19 Df(3R)e-BS2 In(2L)al¹³⁰
Alleles (28)
	al¹³⁰ al^ice al^Scer\UAS.cSa al^ush al^X21 Avic\GFP^{hs.T:Hsap\MYC} Avic\GFP^{Scer\UAS.T:Hsap\MYC,T:SV40\nls2} B-H2^P058 bowl¹ btl::Egfr^{Scer\UAS.T:λ\cI-DD} C15¹ C15² C15³ Dl⁰⁵¹⁵¹ Dl^RevF10 Dl^Scer\UAS.cHa Ecol\lacZ^ap-rK568 Egfr^Eal43 Egfr^f24 Egfr^tsla Lim1^R12.4 Lim1^Scer\UAS.cTb N^{intra.GS.Scer\UAS} N^XK11 Scer\GAL4^dpp.blk1 Scer\GAL4^ptc-559.1 Scer\GAL4^{Scer\FRT.Rnor\CD2.αTub84B} T:λ\cI^DD
Constructs (9)
	P{GAL4-dpp.blk1} P{GAL4-αTub84B(FRT.CD2).P} P{hsp70-Myc-GFP} P{Scer\UAS(-FRT)al.S} P{UAS-Dl.H} P{UAS-Egfr.λtop} P{UAS-GFP.T:Myc.T:nls2} P{UAS-Lim1.Th} P{UAS-N.intra.GS}
Genes (18)
	al ap Avic\GFP B B-H2 bowl btl::Egfr C15 Dl Ecol\lacZ Egfr Lim1 N nub Scer\GAL4 T:Hsap\MYC T:SV40\nls2 T:λ\cI
Insertions (5)
	P{GawB}ptc^559.1 P{lacW}RpI135^X21 P{lArB}B-H2^P058 P{PZ}ap^rK568 P{PZ}Dl⁰⁵¹⁵¹