Reference Report

Reference
Citation	Padash Barmchi, M., Rogers, S., Hacker, U. (2005). DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo. J. Cell Biol. 168(4): 575--585. (Export to RIS)
FlyBase ID	FBrf0184065
Publication Type	Research paper
PubMed ID	15699213
PubMed Abstract	Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family. Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation. We show that the guanine triphosphate (GTP) exchange factor DRhoGEF2 is apically localized in epithelial cells throughout embryogenesis. We demonstrate that DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation, recruits Rho1 to actin rings and regulates actin distribution and actomyosin contractility during nuclear divisions, pole cell formation, and cellularization of syncytial blastoderm embryos. We propose that DRhoGEF2 activity coordinates contractile actomyosin forces throughout morphogenesis in Drosophila by regulating the association of myosin with actin to form contractile cables. Our results support the hypothesis that specific aspects of Rho1 function are regulated by specific GTP exchange factors.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (1)
	Df(3R)tll-e
Alleles (9)
	dia⁵ Rho1^L3 RhoGEF2^3w18 RhoGEF2^4.1 RhoGEF2⁰⁴²⁹¹ RhoGEF2^RE.Scer\UAS Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Scer\GAL4^prd.RG1 sqh^{RLC.T:Avic\GFP-S65T}
Constructs (5)
	P{GAL4-prd.F} P{matα4-GAL-VP16} P{sqh-GFP.RLC} P{UAS-DRhoGEF2.RE} PBac{GAL4D,EYFP}
Genes (14)
	Act5C Act42A Act57B Act79B Act87E Act88F bnk dia kst pnut Rho1 RhoGEF2 Scer\GAL4 sqh
Insertions (1)
	PBac{GAL4D,EYFP}Rho1^L3
Natural transposons (1)
	P-element