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Citation
Supatto, W., Debarre, D., Moulia, B., Brouzes, E., Martin, J.L., Farge, E., Beaurepaire, E. (2005). In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses.  Proc. Natl. Acad. Sci. U.S.A. 102(4): 1047--1052.
FlyBase ID
FBrf0184233
Publication Type
Research paper
Abstract

The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.

PubMed ID
PubMed Central ID
PMC545833 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Alleles (2)
    Genes (3)
    Transgenic Constructs (2)