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Citation
Reinking, J., Lam, M.M., Pardee, K., Sampson, H.M., Liu, S., Yang, P., Williams, S., White, W., Lajoie, G., Edwards, A., Krause, H.M. (2005). The Drosophila nuclear receptor e75 contains heme and is gas responsive.  Cell 122(2): 195--207.
FlyBase ID
FBrf0187235
Publication Type
Research paper
Abstract

Nuclear receptors are a family of transcription factors with structurally conserved ligand binding domains that regulate their activity. Despite intensive efforts to identify ligands, most nuclear receptors are still "orphans." Here, we demonstrate that the ligand binding pocket of the Drosophila nuclear receptor E75 contains a heme prosthetic group. E75 absorption spectra, resistance to denaturants, and effects of site-directed mutagenesis indicate a single, coordinately bound heme molecule. A correlation between the levels of E75 expression and the levels of available heme suggest a possible role as a heme sensor. The oxidation state of the heme iron also determines whether E75 can interact with its heterodimer partner DHR3, suggesting an additional role as a redox sensor. Further, the E75-DHR3 interaction is also regulated by the binding of NO or CO to the heme center, suggesting that E75 may also function as a diatomic gas sensor. Possible mechanisms and roles for these interactions are discussed.

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Related Publication(s)
Note

Powered by gas--a ligand for a fruit fly nuclear receptor.
Thummel, 2005, Cell 122(2): 151--153 [FBrf0187240]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Alleles (1)
    Genes (7)
    Natural transposons (1)
    Experimental Tools (4)
    Transgenic Constructs (1)