Reference Report

Reference
Citation	Myat, M.M., Lightfoot, H., Wang, P., Andrew, D.J. (2005). A molecular link between FGF and Dpp signaling in branch-specific migration of the Drosophila trachea. Dev. Biol. 281(1): 38--52. (Export to RIS)
FlyBase ID	FBrf0187390
Publication Type	Research paper
PubMed ID	15848387
PubMed Abstract	The tracheal system of Drosophila embryos achieves its archetypal branching pattern through a series of cell migration events requiring the FGF, Dpp, and Wg/WNT signaling pathways. To gain insight into tracheal cell migration, we performed an F4 EMS mutagenesis screen to generate and characterize new mutations resulting in tracheal defects. From 2591 mutagenized third chromosome lines, we identified 33 mutations with defects in tracheal development, corresponding to 12 distinct complementation groups. The new mutations included novel hypomorphic alleles of the FGF receptor gene, breathless, and the ETS-domain transcription factor gene, pointed. We show that reduced function of either breathless or pointed specifically affects migration of the dorsal and ventral tracheal branches, more specific functions than previously described for these genes. Our analysis reveals that breathless and pointed control dorsal branch migration through transcriptional regulation of the Dpp pathway effectors, Knirps and Knirps-related, which are necessary for migration of this branch. We further show that expression of knirps or knirps-related rescues dorsal but not ventral branch migration in the breathless hypomorph. These studies support a model in which both the Dpp- and the FGF-signaling pathways control expression of knirps and knirps-related, thereby regulating cell migration during dorsal branch formation.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (34)
	Df(3L)66C-G28 Df(3L)AC1 Df(3L)Ar14-8 Df(3L)brm11 Df(3L)emc-E12 Df(3L)fz-GF3b Df(3L)fz-M21 Df(3L)GN24 Df(3L)h-i22 Df(3L)kto2 Df(3L)M21 Df(3L)ME107 Df(3L)pbl-X1 Df(3L)Pc-2q Df(3L)R-G7 Df(3L)ri-79c Df(3L)st-k3 Df(3L)vin5 Df(3L)vin7 Df(3L)VW3 Df(3L)XS533 Df(3L)ZN47 Df(3R)3450 Df(3R)Antp17 Df(3R)Dl-BX12 Df(3R)Dr-rv1 Df(3R)e1025-14 Df(3R)Espl3 Df(3R)mbc-30 Df(3R)mbc-R1 Df(3R)M-Kx1 Df(3R)p-XT103 Df(3R)Scr Tp(3;2)by62
Alleles (52)
	aul⁵⁸⁹ aul³⁹⁹⁹ bnl⁷⁰⁸ bnl⁰⁰⁸⁵⁷ bnl^Scer\UAS.cSa btl⁹⁵ btl⁴⁶⁹ btl¹⁹²⁸ btl^dev1 btl^H82Δ3 btl^H82Δ11 btl^LG19 btl^Scer\UAS.cDa Ecol\lacZ^sano-rp395 fang⁵⁸⁶ gim²³⁸⁸ gloin⁹⁹ gloin³¹⁹ gloin⁴²⁷ gloin¹⁰⁴¹ gloin¹⁰⁴³ gloin¹²⁷³ gloin¹⁷²¹ gloin¹⁷⁸⁶ gloin¹⁹⁸⁶ gloin²⁰⁰¹ gloin²⁰⁰⁹ gloin²⁰⁵⁶ h⁶⁷⁴ hh²¹ hh⁵²⁰ hh⁶⁸⁸ klar²⁶⁷⁹ kni⁶ kni^Scer\UAS.cCa knrl^Scer\UAS.cLa pnt⁷³⁷ pnt^Δ88 put¹⁰⁴⁶⁰ rho²⁰⁷ rho^PΔ38 Scer\GAL4^69B Scer\GAL4^btl.PS stumps⁶⁵ stumps⁸⁷⁸ stumps¹⁴⁶⁸ stumps¹⁶⁵³ stumps¹⁸⁸⁷ stumps²⁴⁰⁷ stumps²⁷⁵⁶ tkv^QD.Scer\UAS tkv^Scer\UAS.cNa
Constructs (7)
	P{GAL4-btl.S} P{UAS-bnl.S} P{UAS-btl.D} P{UAS-kni.C} P{UAS-knrl.L} P{UAS-tkv.Na} P{UAS-tkv.QD}
Genes (21)
	aul bnl btl crb dpp Ecol\lacZ fang gim gloin h hh klar kni knrl pnt put rho Scer\GAL4 stumps tkv trh
Insertions (6)
	P{5'lwB}btl^H82Δ3 P{5'lwB}btl^H82Δ11 P{GawB}69B P{lacZ}sano^rp395 P{PZ}bnl⁰⁰⁸⁵⁷ P{PZ}put¹⁰⁴⁶⁰