Reference Report

Reference
Citation	Medioni, C., Noselli, S. (2005). Dynamics of the basement membrane in invasive epithelial clusters in Drosophila. Development 132(13): 3069--3077. (Export to RIS)
FlyBase ID	FBrf0187473
Publication Type	Research paper
PubMed ID	15944190
PubMed Abstract	The basement membrane (BM) represents a barrier to cell migration, which has to be degraded to promote invasion. However, the role and behaviour of the BM during the development of pre-invasive cells is only poorly understood. Drosophila border cells (BCs) provide an attractive genetic model in which to study the cellular mechanisms underlying the migration of mixed cohorts of epithelial cells. BCs are made of two different epithelial cell types appearing sequentially during oogenesis: the polar cells and the outer BCs. Here, we show that the pre-invasive polar cells undergo an unusual and asymmetrical apical capping with major basement membrane proteins, including the two Drosophila Collagen IV alpha chains, Laminin A and Perlecan. Capping of polar cells proceeds through a novel, basal-to-apical transcytosis mechanism that involves the small GTPase Drab5. Apical capping is transient and is followed by rapid shedding prior to the initiation of BC migration, suggesting that the apical cap blocks migration. Consistently, non-migratory polar cells remain capped. We further show that JAK/STAT signalling and recruitment of outer BCs are required for correct shedding and migration. The dynamics of the BM represents a marker of migratory BC, revealing a novel developmentally regulated behaviour of BM coupled to epithelial cell invasiveness.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (47)
	aos^Scer\UAS.cMa Avic\GFP^trol-658 Avic\GFP^trol-1700 Avic\GFP^trol-1973 Avic\GFP^trol-2840 Avic\GFP^trol-2867 Avic\GFP^trol-G6 Avic\GFP^vkg-G205 Avic\GFP^vkg-G454 Cdc42^F89.Scer\UAS Cdc42^N17.Scer\UAS Cdc42^V12.Scer\UAS dome^{Scer\UAS.cCGHa} dome^{ΔCYT.Scer\UAS} dome^{ΔEXT.Scer\UAS} dome^{ΔTMCYT.Scer\UAS} dor^{1-1002.Scer\UAS} EcR^B1.Scer\UAS EcR^B2.Scer\UAS EcR^C.Scer\UAS Egfr^Scer\UAS.cBa eya^Scer\UAS.cBa eya^Scer\UAS.cBb eya^unspecified InR^Scer\UAS.cHa Mmp1^f1.Scer\UAS Mmp1^f2.Scer\UAS Mmp2^Scer\UAS.cPa os^Scer\UAS.cZa Rab5^{S43N.Scer\UAS} Rab5^Scer\UAS.cEa Rab7^{Q67L.Scer\UAS} Rac1^L89.Scer\UAS Rac1^N17.Scer\UAS Rac1^V12.Scer\UAS RhoL^N25.Scer\UAS Scer\GAL4^slbo.2.6 Scer\GAL4^upd shi^{K44A.Scer\UAS} Timp^Scer\UAS.cPa trol⁶⁵⁸ trol¹⁷⁰⁰ trol¹⁹⁷³ trol²⁸⁴⁰ trol²⁸⁶⁷ trol^G6 vkg^G205
Constructs (31)
	P{GAL4-slbo.2.6} P{UAS-argos.M} P{UAS-Cdc42.F89} P{UAS-Cdc42.N17} P{UAS-Cdc42.V12} P{UAS-dome.CGH} P{UAS-dome.ΔCYT} P{UAS-dome.ΔEXT} P{UAS-dome.ΔTMCYT} P{UAS-dor.1-1002} P{UAS-EcR.B1} P{UAS-EcR.B2} P{UAS-EcR.C} P{UAS-Egfr.B} P{UAS-eya.B.I} P{UAS-eya.B.II} P{UAS-InR.H} P{UAS-Mmp1.f1} P{UAS-Mmp1.f2} P{UAS-Mmp2.P} P{UAS-os.Z} P{UAS-Rab5.E} P{UAS-Rab5.S43N} P{UAS-Rab7.Q67L} P{UAS-Rac1.L89} P{UAS-Rac1.N17} P{UAS-Rac1.V12} P{UAS-RhoL.N25} P{UAS-shi.K44A} P{UAS-Timp.P} P{upd-GAL4}
Genes (25)
	aos Avic\GFP Cdc42 Cg25C crb dome dor EcR Egfr eya Fas2 InR LanA Mmp1 Mmp2 os Rab5 Rab7 Rac1 RhoL Scer\GAL4 shi Timp trol vkg
Insertions (8)
	P{PTT-un1}trol⁶⁵⁸ P{PTT-un1}trol¹⁷⁰⁰ P{PTT-un1}trol¹⁹⁷³ P{PTT-un1}trol²⁸⁴⁰ P{PTT-un1}trol²⁸⁶⁷ P{PTT-un1}trol^G6 P{PTT-un1}vkg^G205 P{PTT-un1}vkg^G454
Natural transposons (1)
	P-element