Reference Report

Reference
Citation	Pappu, K.S., Ostrin, E.J., Middlebrooks, B.W., Sili, B.T., Chen, R., Atkins, M.R., Gibbs, R., Mardon, G. (2005). Dual regulation and redundant function of two eye-specific enhancers of the Drosophila retinal determination gene dachshund. Development 132(12): 2895--2905. (Export to RIS)
FlyBase ID	FBrf0187476
Publication Type	Research paper
PubMed ID	15930118
PubMed Abstract	Drosophila eye development is controlled by a conserved network of retinal determination (RD) genes. The RD genes encode nuclear proteins that form complexes and function in concert with extracellular signal-regulated transcription factors. Identification of the genomic regulatory elements that govern the eye-specific expression of the RD genes will allow us to better understand how spatial and temporal control of gene expression occurs during early eye development. We compared conserved non-coding sequences (CNCSs) between five Drosophilids along the approximately 40 kb genomic locus of the RD gene dachshund (dac). Our analysis uncovers two separate eye enhancers in intron eight and the 3' non-coding regions of the dac locus defined by clusters of highly conserved sequences. Loss- and gain-of-function analyses suggest that the 3' eye enhancer is synergistically activated by a combination of eya, so and dpp signaling, and only indirectly activated by ey, whereas the 5' eye enhancer is primarily regulated by ey, acting in concert with eya and so. Disrupting conserved So-binding sites in the 3' eye enhancer prevents reporter expression in vivo. Our results suggest that the two eye enhancers act redundantly and in concert with each other to integrate distinct upstream inputs and direct the eye-specific expression of dac.
DOI
Related Publication(s)
Supplementary material	[title not yet available] [FBrf0191587]
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (24)
	Avic\GFP^{dac.3EE.0.85.hs.Stinger} Avic\GFP^{dac.3EE.0.194.hs.Stinger} Avic\GFP^{dac.3EE.0.659.hs.Stinger} Avic\GFP^{hs.Stinger.T:nls-tra} dac³ dac⁷ dac^{Stinger.T:Ivir\HA1} Ecol\lacZ^{dac.3EE.0.39kb} Ecol\lacZ^{dac.3EE.0.85kb} Ecol\lacZ^{dac.3EE.0.194kb} Ecol\lacZ^{dac.3EE.0.659kb} Ecol\lacZ^{dac.3EE.1.9kb} Ecol\lacZ^{dac.5EE.1.7kb} Ecol\lacZ^dac.16.6kb Ecol\lacZ^{dac.S1mut.3EE.0.194kb} Ecol\lacZ^{dac.S2mut.3EE.0.194kb} ey^Scer\UAS.cHa eya² eya^Scer\UAS.cPa Mad^1-2 Scer\GAL4^30A Scer\GAL4^dpp.blk1 so¹ so^Scer\UAS.cPa
Constructs (19)
	P{CaSpeR-AUG-βgal} P{CaSpeR-dac.3EE.0.39kb-βgal} P{CaSpeR-dac.3EE.0.85kb-βgal} P{CaSpeR-dac.3EE.0.194kb-βgal} P{CaSpeR-dac.3EE.0.659kb-βgal} P{CaSpeR-dac.3EE.1.9kb-βgal} P{CaSpeR-dac.5EE-βgal} P{CaSpeR-dac.16.6kb-βgal} P{CaSpeR-dac.S1mut.3EE.0.194kb-betagal} P{CaSpeR-dac.S2mut.3EE.0.194kb-betagal} P{GAL4-dpp.blk1} P{H-Stinger.dac.3EE.0.85} P{H-Stinger.dac.3EE.0.194} P{H-Stinger.dac.3EE.0.659} P{H-Stinger.HA-dac} P{H-Stinger} P{UAS-ey.H} P{UAS-eya.P} P{UAS-so.P}
Genes (19)
	Avic\GFP CG4580 dac Derc\dac dpp Dpse\dac Dvir\dac Dwil\dac Ecol\lacZ ey eya Idgf3 Mad Scer\GAL4 sens so T:Ivir\HA1 T:nls-tra Tpr2
Insertions (1)
	P{GawB}30A
Natural transposons (1)
	P-element
Sequence features (4)
	dac_3EE-16.6kb dac_3EE-194 dac_3EE-390 dac_5EE