Reference Report

Reference
Citation	Silver, D.L., Geisbrecht, E.R., Montell, D.J. (2005). Requirement for JAK/STAT signaling throughout border cell migration in Drosophila. Development 132(15): 3483--3492. (Export to RIS)
FlyBase ID	FBrf0187483
Publication Type	Research paper
PubMed ID	16000386
PubMed Abstract	The evolutionarily conserved JAK/STAT signaling pathway is essential for the proliferation, survival and differentiation of many cells including cancer cells. Recent studies have implicated this transcriptional pathway in the process of cell migration in humans, mice, Drosophila and Dictyostelium. In the Drosophila ovary, JAK/STAT signaling is necessary and sufficient for the specification and migration of a group of cells called the border cells; however, it is not clear to what extent the requirement for cell fate is distinct from that for cell migration. We found that STAT protein is enriched in the migrating border cells throughout their migration and is an indicator of cells with highest JAK/STAT activity. In addition, stat(ts) mutants exhibited border cell migration defects after just 30 minutes at the non-permissive temperature, prior to any detectable change in the expression of cell fate markers. At later times, cell fate changes became evident, indicating that border cell fate is labile. JAK/STAT signaling was also required for organization of the border cell cluster. Finally, we show that both the accumulation of STAT protein and nuclear accumulation are positively regulated by JAK/STAT activity. The activity of the pathway is negatively regulated by overexpression of a SOCS protein and by blocking endocytosis. Together, our findings suggest that the requirement for STAT in border cells extends beyond the initial specification and delamination of cells from the epithelium.
DOI
Related Publication(s)
Supplementary material	Supplemental Figure 1. [FBrf0191759]
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Secondary IDs
Language of Publication	English
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Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (22)
	Avic\GFP^Scer\UAS.cUa dome^{Scer\UAS.T:Avic\GFP-EGFP} dome^{ΔCYT.Scer\UAS} Ecol\lacZ^MA33 Ecol\lacZ^slbo-01310 hop^Scer\UAS.cHa hop^Tum.Scer\UAS os^Scer\UAS.cCa os^TM.Scer\UAS Rac1^N17.Scer\UAS Scer\GAL4^Act5C.PI Scer\GAL4^c306 Scer\GAL4^slbo.2.6 shi^{K44A.Scer\UAS} Socs36E^{Scer\UAS.P\T.cCa} Socs36E^{SH2.Scer\UAS.P\T} Socs36E^{ΔSB.Scer\UAS.P\T} Stat92E³⁹⁷ Stat92E⁰⁶³⁴⁶ Stat92E^EP3391 Stat92E^F T:Avic\GFP^EGFP
Constructs (14)
	P{Act5C-GAL4} P{GAL4-slbo.2.6} P{UAS-dome.EGFP} P{UAS-dome.ΔCYT} P{UAS-GFP.U} P{UAS-hop.H} P{UAS-hop.Tum} P{UAS-os.C} P{UAS-os.TM} P{UASp-Socs36E.C} P{UASp-Socs36E.SH2} P{UASp-Socs36E.ΔSB} P{UAS-Rac1.N17} P{UAS-shi.K44A}
Genes (12)
	arm Avic\GFP dome Ecol\lacZ Fas3 hop os Rac1 Scer\GAL4 shi Socs36E Stat92E
Insertions (4)
	P{EP}Stat92E^EP3391 P{GawB}c306 P{lacZ}MA33 P{PZ}slbo⁰¹³¹⁰
Natural transposons (1)
	P-element