Reference Report

Reference
Citation	Swan, A., Barcelo, G., Schupbach, T. (2005). Drosophila Cks30A interacts with Cdk1 to target Cyclin A for destruction in the female germline. Development 132(16): 3669--3678. (Export to RIS)
FlyBase ID	FBrf0187485
Publication Type	Research paper
PubMed ID	16033797
PubMed Abstract	Cks is a small highly conserved protein that plays an important role in cell cycle control in different eukaryotes. Cks proteins have been implicated in entry into and exit from mitosis, by promoting Cyclin-dependent kinase (Cdk) activity on mitotic substrates. In yeast, Cks can promote exit from mitosis by transcriptional regulation of cell cycle regulators. Cks proteins have also been found to promote S-phase via an interaction with the SCF(Skp2) Ubiquitination complex. We have characterized the Drosophila Cks gene, Cks30A and we find that it is required for progression through female meiosis and the mitotic divisions of the early embryo through an interaction with Cdk1. Cks30A mutants are compromised for Cyclin A destruction, resulting in an arrest or delay at the metaphase/anaphase transition, both in female meiosis and in the early syncytial embryo. Cks30A appears to regulate Cyclin A levels through the activity of a female germline-specific anaphase-promoting complex, CDC20-Cortex. We also find that a second closely related Cks gene, Cks85A, plays a distinct, non-overlapping role in Drosophila, and the two genes cannot functionally replace each other.
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (2)
	Df(2L)30A-C Df(2L)γ25
Alleles (14)
	cdc2^E1-23 cdc2^E1-24 Cks30A^HG24 Cks30A^{K10E.S50E.Scer\UAS.T:Zzzz\FLAG} Cks30A^KO Cks30A^{P61L.Scer\UAS.T:Zzzz\FLAG} Cks30A^RA74 Cks30A^{Scer\UAS.T:Zzzz\FLAG} Cks85A^ex15 Cks85A^{Scer\UAS.T:Zzzz\FLAG} cort^QW55 CycA^C8LR1 Scer\GAL4^da.G32 Scer\GAL4^{nos.UTR.T:Hsim\VP16}
Constructs (6)
	P{GAL4::VP16-nos.UTR} P{GAL4-da.G32} P{UAS-Cks30A-FLAG} P{UAS-Cks85A-FLAG} P{UAS-FLAG-Cks30A.K10E.S50E} P{UAS-FLAG-Cks30A.P61L}
Genes (17)
	cdc2 cdc2c Cks30A Cks85A cnn cort CycA CycB CycB3 decay fzy His3 Lam rap Scer\GAL4 T:Hsim\VP16 T:Zzzz\FLAG
Natural transposons (1)
	P-element