Reference Report

Reference
Citation	Williams, D.W., Truman, J.W. (2005). Cellular mechanisms of dendrite pruning in Drosophila: insights from in vivo time-lapse of remodeling dendritic arborizing sensory neurons. Development 132(16): 3631--3642. (Export to RIS)
FlyBase ID	FBrf0187491
Publication Type	Research paper
PubMed ID	16033801
PubMed Abstract	Regressive events that refine exuberant or inaccurate connections are critical in neuronal development. We used multi-photon, time-lapse imaging to examine how dendrites of Drosophila dendritic arborizing (da) sensory neurons are eliminated during early metamorphosis, and how intrinsic and extrinsic cellular mechanisms control this deconstruction. Removal of the larval dendritic arbor involves two mechanisms: local degeneration and branch retraction. In local degeneration, major branch severing events entail focal disruption of the microtubule cytoskeleton, followed by thinning of the disrupted region, severing and fragmentation. Retraction was observed at distal tips of branches and in proximal stumps after severing events. The pruning program of da neuron dendrites is steroid induced; cell-autonomous dominant-negative inhibition of steroid action blocks local degeneration, although retraction events still occur. Our data suggest that steroid-induced changes in the epidermis may contribute to dendritic retraction. Finally, we find that phagocytic blood cells not only engulf neuronal debris but also attack and sever intact branches that show signs of destabilization.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (9)
	BacA\p35^Scer\UAS.cHa EcR^{B1-ΔC655.W650A.Scer\UAS} His2Av^{T:Avic\GFP-S65T} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^ppk.1.9 Scer\GAL4^smid-C161 T:Avic\GFP^S65C T:Avic\GFP^S65T αTub84B^{Scer\UAS.P\T.T:Avic\GFP-S65C}
Constructs (6)
	P{His2Av^{T:Avic\GFP-S65T}} P{ppk-GAL4.1.9} P{UAS-EcR.B1-ΔC655.W650A} P{UAS-mCD8::GFP.L} P{UAS-p35.H} P{UASp-GFPS65C-αTub84B}
Genes (10)
	BacA\p35 ct EcR futsch His2Av Mmus\Cd8a ppk Scer\GAL4 T:Avic\GFP αTub84B
Insertions (1)
	P{GawB}smid^C161