Reference Report

Reference
Citation	Yao, J.G., Sun, Y.H. (2005). Eyg and Ey Pax proteins act by distinct transcriptional mechanisms in Drosophila development. EMBO J. 24(14): 2602--2612. (Export to RIS)
FlyBase ID	FBrf0187515
Publication Type	Research paper
PubMed ID	15973436
PubMed Abstract	Drosophila has two pairs of Pax genes, ey/toy and eyg/toe, that play different functions during eye development. ey specifies eye fate, while eyg promotes cell proliferation. We have determined the molecular basis for the functional diversity of Eyg and Ey. Eyg and Ey act by distinct transcriptional mechanisms. They use different DNA-binding domains for target recognition. Most interestingly, Eyg acts exclusively as a repressor, whereas Ey is an activator. Several vertebrate Pax proteins are known to switch between activator and repressor activities, but none as repressors only. Eyg may be the first Pax protein as a dedicated repressor. Vertebrates produce a Pax6 isoform, Pax6-5a, differing from Pax6 in DNA-binding properties and functions and structurally similar to Eyg/Toe. We found that Pax6-5a acts as an activator like Ey, but has DNA-binding specificity like Eyg.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Alleles (65)
	Avic\GFP^eyg.PD.en Avic\GFP^Scer\UAS.cUa Avic\GFP^{W9.wg.Scer\UAS} Ecol\lacZ^Dll-01092 Ecol\lacZ^so.10 Ecol\lacZ^wg-en11 ey::eyg^{EEEEEG.Scer\UAS} ey::eyg^{EEEEGE.Scer\UAS} ey::eyg^{EEEGEE.Scer\UAS} ey::eyg^{EEEGEG.Scer\UAS} ey::eyg^{EEEGGG.Scer\UAS} ey::eyg^{EEGEEE.Scer\UAS} ey::eyg^{EEGEGE.Scer\UAS} ey::eyg^{EEGGGE.Scer\UAS} ey::eyg^{EEGGGG.Scer\UAS} ey::eyg^{EGGGGE.Scer\UAS} ey::eyg^{EGGGGG.Scer\UAS} ey::eyg^{GEEEEE.Scer\UAS} ey::eyg^{GEEEEG.Scer\UAS} ey::eyg^{GGEEEE.Scer\UAS} ey::eyg^{GGEEEG.Scer\UAS} ey::eyg^{GGEGEG.Scer\UAS} ey::eyg^{GGEGGG.Scer\UAS} ey::eyg^{GGGEEE.Scer\UAS} ey::eyg^{GGGEGE.Scer\UAS} ey::eyg^{GGGEGG.Scer\UAS} ey::eyg^{GGGGEG.Scer\UAS} ey::eyg^{GGGGGE.Scer\UAS} ey^{Scer\UAS.T:Zzzz\FLAG,T:SV40\nls2} ey^{ΔPAI.Scer\UAS} ey^{ΔRED.Scer\UAS} eyg^20MD1 eyg^22-2 eyg^325.Scer\UAS eyg^438.Scer\UAS eyg^{554.Scer\UAS.T:SV40\nls2} eyg^554.Scer\UAS eyg^643.Scer\UAS eyg^670.Scer\UAS eyg^C.Scer\UAS eyg^en.Scer\UAS eyg^M3-12 eyg^mHD.Scer\UAS eyg^{mPD.mHD.Scer\UAS} eyg^mPD.Scer\UAS eyg^{Scer\UAS.T:Hsim\VP16} eyg^ΔC.Scer\UAS eyg^{ΔHD.Scer\UAS} eyg^{ΔL.ΔRED.Scer\UAS} eyg^{ΔPAI.Scer\UAS} eyg^{ΔPAI.ΔL.Scer\UAS} eyg^{ΔPD.Scer\UAS} eyg^{ΔRED.Scer\UAS} Hsap\PAX6^5a.Scer\UAS Hsap\PAX6^{5a.Scer\UAST:Hsim\VP16} Scer\GAL4^dpp.blk1 Scer\GAL4^en.wg Scer\GAL4^eyg-22-2 Scer\GAL4^eyg.B.wg Scer\GAL4^eyg.C.wg Scer\GAL4^eyg.HD.wg Scer\GAL4^{eyg.mPDmHD.wg} Scer\GAL4^eyg.PD.wg Scer\GAL4^W11.wg w^+mC
Constructs (58)
	P{en-GFP.eyg.PD} P{GAL4-dpp.blk1} P{so-lacZ.10} P{UAS-ey::eyg.EEEEEG} P{UAS-ey::eyg.EEEEGE} P{UAS-ey::eyg.EEEGEE} P{UAS-ey::eyg.EEEGEG} P{UAS-ey::eyg.EEEGGG} P{UAS-ey::eyg.EEGEEE} P{UAS-ey::eyg.EEGEGE} P{UAS-ey::eyg.EEGGGE} P{UAS-ey::eyg.EEGGGG} P{UAS-ey::eyg.EGGGGE} P{UAS-ey::eyg.EGGGGG} P{UAS-ey::eyg.GEEEEE} P{UAS-ey::eyg.GEEEEG} P{UAS-ey::eyg.GGEEEE} P{UAS-ey::eyg.GGEEEG} P{UAS-ey::eyg.GGEGEG} P{UAS-ey::eyg.GGEGGG} P{UAS-ey::eyg.GGGEEE} P{UAS-ey::eyg.GGGEGE} P{UAS-ey::eyg.GGGEGG} P{UAS-ey::eyg.GGGGEG} P{UAS-ey::eyg.GGGGGE} P{UAS-ey.FLAG.nls} P{UAS-ey.ΔPAI} P{UAS-ey.ΔRED} P{UAS-eyg.325} P{UAS-eyg.438} P{UAS-eyg.554.nls} P{UAS-eyg.554} P{UAS-eyg.643} P{UAS-eyg.670} P{UAS-eyg.C} P{UAS-eyg.en} P{UAS-eyg.mHD} P{UAS-eyg.mPD} P{UAS-eyg.mPDmHD} P{UAS-eyg.VP16} P{UAS-eyg.ΔC} P{UAS-eyg.ΔHD} P{UAS-eyg.ΔL.ΔRED} P{UAS-eyg.ΔPAI.ΔL} P{UAS-eyg.ΔPAI} P{UAS-eyg.ΔPD} P{UAS-eyg.ΔRED} P{UAS-GFP.U} P{UAS-GFP.W9.wg} P{UAS-Hsap\PAX6.5a.VP16} P{UAS-PAX6.5a} P{wg-GAL4.en} P{wg-GAL4.eyg.B} P{wg-GAL4.eyg.HD} P{wg-GAL4.eygC} P{wg-GAL4.mPDmHD} P{wg-GAL4.PD} P{wg-GAL4.W11}
Genes (16)
	Avic\GFP Dll Ecol\lacZ elav ey ey::eyg eyg Hsap\PAX6 Scer\GAL4 T:Hsim\VP16 T:SV40\nls2 T:Zzzz\FLAG toe toy w wg
Insertions (5)
	P{en1}wg^en11 P{GawB}eyg^22-2 P{lacW}eyg^M3-12 P{lacZ}eyg^20MD1 P{PZ}Dll⁰¹⁰⁹²
Natural transposons (1)
	P-element