Open Close
Reference
Citation
Couceiro, J.R., Martin-Bermudo, M.D., Bustelo, X.R. (2005). Phylogenetic conservation of the regulatory and functional properties of the Vav oncoprotein family.  Exp. Cell Res. 308(2): 364--380.
FlyBase ID
FBrf0187549
Publication Type
Research paper
Abstract

Vav proteins are phosphorylation-dependent GDP/GTP exchange factors for Rho/Rac GTPases. Despite intense characterization of mammalian Vav proteins both biochemically and genetically, there is little information regarding the conservation of their biological properties in lower organisms. To approach this issue, we have performed a characterization of the regulatory, catalytic, and functional properties of the single Vav family member of Drosophila melanogaster. These analyses have shown that the intramolecular mechanisms controlling the enzyme activity of mammalian Vav proteins are already present in Drosophila, suggesting that such properties have been set up before the divergence between protostomes and deuterostomes during evolution. We also show that Drosophila and mammalian Vav proteins have similar catalytic specificities. As a consequence, Drosophila Vav can trigger oncogenic transformation, morphological change, and enhanced cell motility in mammalian cells. Gain-of-function studies using transgenic flies support the implication of this protein in cytoskeletal-dependent processes such as embryonic dorsal closure, myoblast fusion, tracheal development, and the migration/guidance of different cell types. These results highlight the important roles of Vav proteins in the signal transduction pathways regulating cytoskeletal dynamics. Moreover, they indicate that the foundations for the regulatory and enzymatic activities of this protein family have been set up very early during evolution.

PubMed ID
PubMed Central ID
PMC1447607 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Exp. Cell Res.
    Title
    Experimental Cell Research
    Publication Year
    1950-
    ISBN/ISSN
    0014-4827
    Data From Reference
    Alleles (9)
    Genes (5)
    Insertions (4)
    Experimental Tools (2)
    Transgenic Constructs (5)