Reference Report

Reference
Citation	Heeger, S., Leismann, O., Schittenhelm, R., Schraidt, O., Heidmann, S., Lehner, C.F. (2005). Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog. Genes Dev. 19(17): 2041--2053. (Export to RIS)
FlyBase ID	FBrf0187592
Publication Type	Research paper
PubMed ID	16140985
PubMed Abstract	Faithful transmission of genetic information during mitotic divisions depends on bipolar attachment of sister kinetochores to the mitotic spindle and on complete resolution of sister-chromatid cohesion immediately before the metaphase-to-anaphase transition. Separase is thought to be responsible for sister-chromatid separation, but its regulation is not completely understood. Therefore, we have screened for genetic loci that modify the aberrant phenotypes caused by overexpression of the regulatory separase complex subunits Pimples/securin and Three rows in Drosophila. An interacting gene was found to encode a constitutive centromere protein. Characterization of its centromere localization domain revealed the presence of a diverged CENPC motif. While direct evidence for an involvement of this Drosophila Cenp-C homolog in separase activation at centromeres could not be obtained, in vivo imaging clearly demonstrated that it is required for normal attachment of kinetochores to the spindle.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Aberrations (11)
	Df(2R)vg56 Df(3R)CA1 Df(3R)CA3 Df(3R)D8 Df(3R)dsx11 Df(3R)dsx27 Df(3R)Exel6149 Df(3R)G1 Df(3R)p25 Df(3R)p712 Df(3R)p-XT103
Alleles (55)
	Cap-G⁶ Cenp-C^{1084stop.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^{1192stop.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^+t8.7 Cenp-C^+t14.6 Cenp-C^abcd.m Cenp-C^{C.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^C.T:Hsap\MYC Cenp-C^{CC.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^{CM.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^{CN.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^{FL.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^IR35 Cenp-C^{M.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^{N.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^N.T:Hsap\MYC Cenp-C^prl41 Cenp-C^{R1101G.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^Scer\UAS.cHa Cenp-C^{T:Avic\GFP-EGFP} Cenp-C^{T:Avic\GFP-EYFP} Cenp-C^unspecified Cenp-C^{ΔAT1.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^{ΔAT2.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^{ΔN.Scer\UAS.T:Avic\GFP-EGFP} Cenp-C^{ΔNLS.Scer\UAS.T:Avic\GFP-EGFP} CG11737^t15 CG31259^t15 CG31454^t15 CG31460^t6.4 CG31460^t14.6 cid^MtnA.BLRP cid^MtnA.BLRPmut cid^Scer\UAS.cJa cid^T12-1 cid^T22-4 cid^{T:Avic\GFP-EGFP} His2Av^{T:Disc\RFP-mRFP} mRpS18C^t6.4 mRpS18C^t14.6 pch2^t6.4 pch2^t14.6 pim^{Scer\UAS.T:Hsap\MYC} Scer\GAL4^Act5C.PU Scer\GAL4^Bx-MS1096 Scer\GAL4^GMR.PF Scer\GAL4^hs.2sev Scer\GAL4^sca-537.4 Scer\GAL4^unspecified T:Avic\GFP^EGFP T:Avic\GFP^EYFP T:Disc\RFP^mRFP thr^{Scer\UAS.g.1-930.T:Hsap\MYC} thr^{ΔC.GMR.T:Hsap\MYC} w^+mC
Constructs (28)
	P{Cenp-C.abcd.m} P{Cenp-C.abcd} P{Cenp-C.d} P{CG11737^t15} P{CG31453.abc} P{GAL4-Hsp70.sev} P{GAL4-ninaE.GMR} P{gCenp-C-EGFP} P{gCenp-C-myc} P{gcid-EGFP-cid} P{gEYFP-Cenp-C} P{GMR-thr.ΔC} P{gmyc-Cenp-C} P{His2Av-mRFP1} P{UAS-Cenp-C.H} P{UAS-cid.J} P{UAS-EGFP-Cenp-C.C} P{UAS-EGFP-Cenp-C.CC} P{UAS-EGFP-Cenp-C.CM} P{UAS-EGFP-Cenp-C.CN} P{UAS-EGFP-Cenp-C.FL} P{UAS-EGFP-Cenp-C.M} P{UAS-EGFP-Cenp-C.N} P{UAS-EGFP-Cenp-C.ΔAT1} P{UAS-EGFP-Cenp-C.ΔAT2} P{UAS-EGFP-Cenp-C.ΔN} P{UAS-EGFP-Cenp-C.ΔNLS} P{UAS-pim-myc}
Genes (21)
	BubR1 Cap-G Cenp-C CG11737 CG31259 CG31454 CG31460 cid CycB His2Av mRpS18C Or85b Or85c Or85d pch2 pim Scer\GAL4 T:Hsap\MYC T:Zzzz\BLRP thr w
Insertions (2)
	P{GawB}Bx^MS1096 P{GawB}sca^537.4
Natural transposons (1)
	P-element