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Citation
Jiang, F., Ye, X., Liu, X., Fincher, L., McKearin, D., Liu, Q. (2005). Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila.  Genes Dev. 19(14): 1674--1679.
FlyBase ID
FBrf0187593
Publication Type
Research paper
Abstract

In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNA-binding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.

PubMed ID
PubMed Central ID
PMC1176004 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Dev.
    Title
    Genes & Development
    Publication Year
    1987-
    ISBN/ISSN
    0890-9369
    Data From Reference
    Alleles (1)
    Genes (11)
    Physical Interactions (10)
    Cell Lines (1)