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Kolesnikova, T.D., Makunin, I.V., Volkova, E.I., Pirrotta, V., Belyaeva, E.S., Zhimulev, I.F. (2005). Functional dissection of the suppressor of underreplication protein of Drosophila melanogaster: identification of domains influencing chromosome binding and DNA replication.  Genetica 124(2-3): 187--200.
FlyBase ID
FBrf0187619
Publication Type
Research paper
Abstract

The Suppressor of UnderReplication (SuUR) gene controls the DNA underreplication in intercalary and pericentric heterochromatin of Drosophila melanogaster salivary gland polytene chromosomes. In the present work, we investigate the functional importance of different regions of the SUUR protein by expressing truncations of the protein in an UAS-GAL4 system. We find that SUUR has at least two separate chromosome-binding regions that are able to recognize intercalary and pericentric heterochromatin specifically. The C-terminal part controls DNA underreplication in intercalary heterochromatin and partially in pericentric heterochromatin regions. The C-terminal half of SUUR suppresses endoreplication when ectopically expressed in the salivary gland. Ectopic expression of the N-terminal fragments of SUUR depletes endogenous SUUR from polytene chromosomes, causes the SuUR- phenotype and induces specific swellings in heterochromatin.

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    Language of Publication
    English
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    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetica
    Title
    Genetica
    Publication Year
    1919-
    ISBN/ISSN
    0016-6707
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