Reference Report

Reference
Citation	Radford, S.J., Goley, E., Baxter, K., McMahan, S., Sekelsky, J. (2005). Drosophila ERCC1 Is Required for a Subset of MEI-9-Dependent Meiotic Crossovers. Genetics 170(4): 1737--1745. (Export to RIS)
FlyBase ID	FBrf0187655
Publication Type	Research paper
PubMed ID	15944364
PubMed Abstract	Drosophila MEI-9 is the catalytic subunit of a DNA structure-specific endonuclease required for nucleotide excision repair (NER). The enzymatic activity of this endonuclease during NER requires the presence of a second, noncatalytic subunit called ERCC1. In addition to its role in NER, MEI-9 is required for the generation of most meiotic crossovers. To better understand the role of MEI-9 in crossover formation, we report here the characterization of the Drosophila Ercc1 gene. We created an Ercc1 mutant through homologous gene targeting. We find that Ercc1 mutants are identical to mei-9 mutants in sensitivity to DNA-damaging agents, but have a less severe reduction in the number of meiotic crossovers. MEI-9 protein levels are reduced in Ercc1 mutants; however, overexpression of MEI-9 is not sufficient to restore meiotic crossing over in Ercc1 mutants. We conclude that MEI-9 can generate some meiotic crossovers in an ERCC1-independent manner.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (2)
	Df(2R)knSA3 Dp(3;3)Ercc1^Xdup
Alleles (11)
	Ciao1^t4.7 Ercc1^{X.Scer\SceI.RS} Ercc1^X Ercc1^Xdup HPS1^t4.7 mei-9^A2 mei-9^{Ubi.T:Zzzz\FLAG} mei-9^unspecified mus312^unspecified SMC2^t4.7 w^+mC
Constructs (2)
	P{(FRT.Ercc1^X)} P{Ubi-mei-9.FLAG}
Genes (8)
	Ciao1 Ercc1 HPS1 mei-9 mus312 SMC2 T:Zzzz\FLAG w
Natural transposons (1)
	P-element