Reference Report

Reference
Citation	Schulze, S.R., Sinclair, D.A.R., Fitzpatrick, K.A., Honda, B.M. (2005). A genetic and molecular characterization of two proximal heterochromatic genes on chromosome 3 of Drosophila melanogaster. Genetics 169(4): 2165--2177. (Export to RIS)
FlyBase ID	FBrf0187658
Publication Type	Research paper
PubMed ID	15687284
PubMed Abstract	Heterochromatin comprises a transcriptionally repressive chromosome compartment in the eukaryotic nucleus; this is exemplified by the silencing effect it has on euchromatic genes that have been relocated nearby, a phenomenon known as position-effect variegation (PEV), first demonstrated in Drosophila melanogaster. However, the expression of essential heterochromatic genes within these apparently repressive regions of the genome presents a paradox, an understanding of which could provide key insights into the effects of chromatin structure on gene expression. To date, very few of these resident heterochromatic genes have been characterized to any extent, and their expression and regulation remain poorly understood. Here we report the cloning and characterization of two proximal heterochromatic genes in D. melanogaster, located deep within the centric heterochromatin of the left arm of chromosome 3. One of these genes, RpL15, is uncharacteristically small, is highly expressed, and encodes an essential ribosomal protein. Its expression appears to be compromised in a genetic background deficient for heterochromatin protein 1 (HP1), a protein associated with gene silencing in these regions. The second gene in this study, Dbp80, is very large and also appears to show a transcriptional dependence upon HP1; however, it does not correspond to any known lethal complementation group and is likely to be a nonessential gene.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (4)
	Df(3L)9-56 Df(3L)K-2 Df(3L)Snap25³ In(3L)C90
Alleles (29)
	Dbp80^{dsRNA.Scer\UAS} N^55e11 RpL15³ RpL15^7-1 RpL15^7-1-Rev7 RpL15^7-1-Rev14 RpL15^8-1 RpL15^8-1-Rev3 RpL15^8-1-Rev7 RpL15⁷² RpL15^cDNA.hs RpL15^{cDNA.Scer\UAS} RpL15^gen.Scer\UAS RpL15^PΔ2 RpL15^PΔ2-Rev2 RpL15^PΔ2-Rev5 RpL15^PΔ2-Rev12 RpL15^PΔ2-Rev13 RpL15^PΔ8 RpL15^PΔ8-Rev5 RpL15^PΔ8-Rev12 RpL15^unspecified Scer\GAL4^Act5C.PU Scer\GAL4^hs.PB Snap25^1-16-0 Su(var)205¹ Su(var)205⁴ Su(var)205¹⁴⁹ w^+mC
Constructs (4)
	P{hs-RpL15.cDNA} P{UAS-Dbp80.RNAi} P{UAS-RpL15.cDNA} P{UAS-RpL15.gen}
Genes (9)
	Dbp80 l(3)80Fj N RpL15 Scer\GAL4 Snap25 Su(var)205 vtd w
Insertions (5)
	P{}RpL15^7-1 P{}RpL15^8-1 P{}RpL15^PΔ2 P{}RpL15^PΔ8 P{UAS-RpL15.cDNA}24-1