Reference Report

Reference
Citation	Singh, A., Chan, J., Chern, J.J., Choi, K.W. (2005). Genetic interaction of Lobe with its modifiers in dorsoventral patterning and growth of the Drosophila eye. Genetics 171(1): 169--183. (Export to RIS)
FlyBase ID	FBrf0187660
Publication Type	Research paper
External Crossreferences
PubMed ID	15976174
PubMed Abstract	Dorsoventral (DV) patterning is essential for growth of the Drosophila eye. Recent studies suggest that ventral is the default state of the early eye, which depends on Lobe (L) function, and that the dorsal fate is established later by the expression of the dorsal selector gene pannier (pnr). However, the mechanisms of regulatory interactions between L and dorsal genes are not well understood. For studying the mechanisms of DV patterning in the early eye disc, we performed a dominant modifier screen to identify additional genes that interact with L. The criterion of the dominant interaction was either enhancement or suppression of the L ventral eye loss phenotype. We identified 48 modifiers that correspond to 16 genes, which include fringe (fng), a gene involved in ventral eye patterning, and members of both Hedgehog (Hh) and Decapentaplegic (Dpp) signaling pathways, which promote L function in the ventral eye. Interestingly, 29% of the modifiers (6 enhancers and 9 suppressors) identified either are known to interact genetically with pnr or are members of the Wingless (Wg) pathway, which acts downstream from pnr. The detailed analysis of genetic interactions revealed that pnr and L mutually antagonize each other during second instar of larval development to restrict their functional domains in the eye. This time window coincides with the emergence of pnr expression in the eye. Our results suggest that L function is regulated by multiple signaling pathways and that the mutual antagonism between L and dorsal genes is crucial for balanced eye growth.
BIOSIS ID	2006.20826
Zoological Record ID	14202008887
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FB2011_10
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Editors
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (4)
	Df(1)B Df(2L)BSC7 Df(2R)trix Df(3L)iro-DFM3
Alleles (70)
	aop^EP598 aop^EP2500 ara^Scer\UAS.cGa Avic\GFP^Scer\UAS.cUa B¹ CBP^EP1643 CG3308^EP1230 CG7920^EP3096 CG12054^EP3309 CG16936^EP2452 CG42709^EP3622 Cul-4^EP2518 Cul-4^unspecified Dad^EP3196 dally^EP3168 dco^EP3280 emc¹² emc^EP415 emc^EP3166 emc^EP3614 emc^EP3620 EP950^EP950 EP1324^EP1324 EP1350^EP1350 EP1410^EP1410 EP2012^EP2012 EP2031^EP2031 EP2370^EP2370 EP3087^EP3087 EP3123^EP3123 esg⁰⁵⁷²⁹ esg^EP633 esg^EP683 esg^EP2159 esg^EP2408 fng^EP3082 hh^bar3 hh^EP3521 Hmt-1^EP3387 kek1^EP655 L² L^rev6-3 L^si mir-278^EP1229 mirr^Scer\UAS.cMa nej^EP1149 nej^EP1179 NFAT^EP1353 NFAT^EP1508 noc^EP2173 nrv1^EP2454 pnr^D4.Scer\UAS pnr^{EnR.Scer\UAS.T:en-Rep} pnr^VX6 pygo^unspecified ras^EP1235 ras^EP1427 RhoGAP68F^EP3152 rod^EP3408 Scer\GAL4^bi-omb-Gal4 Scer\GAL4^ey.PH Scer\GAL4^pnr-MD237 sgg¹ sgg^EP1576 smo^EP1220 T:en^Rep TfIIA-S^EP3285 ush^Scer\UAS.cFa w^B1-12 wun^EP2208
Constructs (7)
	P{GAL4-ey.H} P{UAS-ara.G} P{UAS-GFP.U} P{UAS-mirr.M} P{UAS-pnr.D4} P{UAS-pnr.EnR} P{UAS-ush.F}
Genes (60)
	aop ara Avic\GFP B B-H1 B-H2 CBP CG3308 CG6664 CG7920 CG12054 CG16700 CG16936 CG42709 Cul-4 Dad dally dco dlg1 elav emc EP950 EP1324 EP1350 EP1410 EP2012 EP2031 EP2370 EP3087 EP3123 esg ey fng futsch GstS1 hh Hmt-1 kek1 L mir-278 mirr nej NFAT noc nrv1 pnr pygo ras RhoGAP68F rod Scer\GAL4 sens sgg smo TfIIA-S ush w wg wun wun2
Insertions (51)
	P{EP}aop^EP598 P{EP}aop^EP2500 P{EP}CBP^EP1643 P{EP}CG3308^EP1230 P{EP}CG7920^EP3096 P{EP}CG12054^EP3309 P{EP}CG16936^EP2452 P{EP}CG42709^EP3622 P{EP}Cul-4^EP2518 P{EP}Dad^EP3196 P{EP}dally^EP3168 P{EP}dco^EP3280 P{EP}emc^EP415 P{EP}emc^EP3087 P{EP}emc^EP3166 P{EP}emc^EP3614 P{EP}emc^EP3620 P{EP}EP1324^EP1324 P{EP}EP1350^EP1350 P{EP}EP2012^EP2012 P{EP}EP2031^EP2031 P{EP}EP2370^EP2370 P{EP}EP3123^EP3123 P{EP}esg^EP633 P{EP}esg^EP683 P{EP}esg^EP2159 P{EP}esg^EP2408 P{EP}fng^EP3082 P{EP}hh^EP3521 P{EP}Hmt-1^EP3387 P{EP}kek1^EP655 P{EP}mir-278^EP1229 P{EP}nej^EP950 P{EP}nej^EP1149 P{EP}nej^EP1179 P{EP}nej^EP1410 P{EP}NFAT^EP1353 P{EP}NFAT^EP1508 P{EP}noc^EP2173 P{EP}nrv1^EP2454 P{EP}ras^EP1235 P{EP}ras^EP1427 P{EP}RhoGAP68F^EP3152 P{EP}rod^EP3408 P{EP}sgg^EP1576 P{EP}smo^EP1220 P{EP}TfIIA-S^EP3285 P{EP}wun^EP2208 P{GAL4}bi^omb-Gal4 P{GawB}pnr^MD237 P{lacW}mirr^B1-12