Birch-Machin, I., Gao, S., Huen, D., McGirr, R., White, R.A., Russell, S. (2005). Genomic analysis of heat-shock factor targets in Drosophila. Genome Biol. 6(7): R63.
FlyBase ID
FBrf0187687
Publication Type
Research paper
Abstract
We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.
