Reference Report

Reference
Citation	Cole, S.H., Carney, G.E., McClung, C.A., Willard, S.S., Taylor, B.J., Hirsh, J. (2005). Two functional but noncomplementing Drosophila tyrosine decarboxylase genes. J. Biol. Chem. 280(15): 14948--14955. (Export to RIS)
FlyBase ID	FBrf0187786
Publication Type	Research paper
PubMed ID	15691831
PubMed Abstract	The trace biogenic amine tyramine is present in the nervous systems of animals ranging in complexity from nematodes to mammals. Tyramine is synthesized from tyrosine by the enzyme tyrosine decarboxylase (TDC), a member of the aromatic amino acid family, but this enzyme has not been identified in Drosophila or in higher animals. To further clarify the roles of tyramine and its metabolite octopamine, we have cloned two TDC genes from Drosophila melanogaster, dTdc1 and dTdc2. Although both gene products have TDC activity in vivo, dTdc1 is expressed nonneurally, whereas dTdc2 is expressed neurally. Flies with a mutation in dTdc2 lack neural tyramine and octopamine and are female sterile due to egg retention. Although other Drosophila mutants that lack octopamine retain eggs completely within the ovaries, dTdc2 mutants release eggs into the oviducts but are unable to deposit them. This specific sterility phenotype can be partially rescued by driving the expression of dTdc2 in a dTdc2-specific pattern, whereas driving the expression of dTdc1 in the same pattern results in a complete rescue. The disparity in rescue efficiencies between the ectopically expressed Tdc genes may reflect the differential activities of these gene products. The egg retention phenotype of the dTdc2 mutant and the phenotypes associated with ectopic dTdc expression contribute to a model in which octopamine and tyramine have distinct and separable neural activities.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Biol. Chem.
Title	Journal of Biological Chemistry
Publication Year	1905-
ISBN/ISSN	0021-9258
Data from Reference
Aberrations (13)
	Df(2R)42 Df(2R)cn9 Df(2R)cn87e Df(2R)cn88b Df(2R)Drl^rv17 Df(2R)Drl^rv25 Df(2R)nap1 Df(2R)nap9 Df(2R)nap14 Df(2R)nap19 Df(2R)pk78k Df(2R)pk78s Df(2R)ST1
Alleles (9)
	Avic\GFP^{Scer\UAS.T:SV40\nls2} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^Tdc1.PC Scer\GAL4^Tdc2.PC Tbh^nM18 Tdc1^Scer\UAS.cCa Tdc2^RO54 Tdc2^Scer\UAS.cCb w^+mC
Constructs (6)
	P{Tdc1-GAL4.C} P{Tdc2-GAL4.C} P{UAS-GFP.nls} P{UAS-mCD8::GFP.L} P{UAS-Tdc1.C} P{UAS-Tdc2.C}
Genes (12)
	Avic\GFP CG17266 Hsepi Mmus\Cd8a Mob4 Scer\GAL4 T:Avic\GFP T:SV40\nls2 Tbh Tdc1 Tdc2 w
Natural transposons (1)
	P-element