Reference Report

Reference
Citation	Giagtzoglou, N., Koumbanakis, K.A., Fullard, J., Zarifi, I., Delidakis, C. (2005). Role of the Sc C terminus in transcriptional activation and E(spl) repressor recruitment. J. Biol. Chem. 280(2): 1299--1305. (Export to RIS)
FlyBase ID	FBrf0187787
Publication Type	Research paper
PubMed ID	15507447
PubMed Abstract	Neurogenesis in all animals is triggered by the activity of a group of basic helix-loop-helix transcription factors, the proneural proteins, whose expression endows ectodermal regions with neural potential. The eventual commitment to a neural precursor fate involves the interplay of these proneural transcriptional activators with a number of other transcription factors that fine tune transcriptional responses at target genes. Most prominent among the factors antagonizing proneural protein activity are the HES basic helix-loop-helix proteins. We have previously shown that two HES proteins of Drosophila, E(spl)mgamma and E(spl)m7, interact with the proneural protein Sc and thereby get recruited onto Sc target genes to repress transcription. Using in vivo and in vitro assays we have now discovered an important dual role for the Sc C-terminal domain. On one hand it acts as a transcription activation domain, and on the other it is used to recruit E(spl) proteins. In vivo, the Sc C-terminal domain is required for E(spl) recruitment in an enhancer context-dependent fashion, suggesting that in some enhancers alternative interaction surfaces can be used to recruit E(spl) proteins.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Biol. Chem.
Title	Journal of Biological Chemistry
Publication Year	1905-
ISBN/ISSN	0021-9258
Data from Reference
Alleles (29)
	E(spl)m3-HLH^Act5C.PG E(spl)m5-HLH^Act5C.PG E(spl)m7-HLH^Act5C.PG E(spl)m7-HLH^{Act5C.T:Hsim\VP16} E(spl)m7-HLH^KNEQ.Act5C E(spl)m7-HLH^{KNEQAct.Scer\UAS.T:Hsim\VP16} E(spl)m7-HLH^ΔW.Act5C E(spl)m8-HLH^Act5C.PG E(spl)mβ-HLH^Act5C.PG E(spl)mγ-HLH^Act5C.PG E(spl)mδ-HLH^Act5C.PG Ecol\lacZ^ac.0.8 Ecol\lacZ^miniEx4.E1 Ppyr\LUC^{5x.Scer\UAS.Hsim\TK} sc^{1-260.Scer\UAS} sc^{1-290.Scer\UAS} sc^{1-320.Act5C.T:Scer\GAL4} sc^{1-345.Act5C.T:Scer\GAL4} sc^{164-320.Act5C.T:Scer\GAL4} sc^{164-345.Act5C.T:Scer\GAL4} sc^{261-320.Act5C.T:Scer\GAL4} sc^{261-345.Act5C.T:Scer\GAL4} sc^{291-345.Act5C.T:Scer\GAL4} sc^{321-345.Act5C.T:Scer\GAL4} sc^Scer\UAS.cPa Scer\GAL4^{Act5C.G.T:Hsim\VP16} Scer\GAL4^GDBD.Act5C Scer\GAL4^pnr-MD237 w^+mC
Constructs (6)
	P{0.8ac-lacZ} P{m7^KNEQAct} P{sc-lacZ.miniEx4.E1} P{UAS-sc.1-260} P{UAS-sc.1-290} P{UAS-sc.P}
Genes (17)
	ac da E(spl)m3-HLH E(spl)m5-HLH E(spl)m7-HLH E(spl)m8-HLH E(spl)mβ-HLH E(spl)mγ-HLH E(spl)mδ-HLH Ecol\lacZ pnr Ppyr\LUC sc Scer\GAL4 T:Hsim\VP16 T:Scer\GAL4 w
Insertions (1)
	P{GawB}pnr^MD237
Transcripts (3)
	Ecol\lacZ^ac.0.8RA Ecol\lacZ^miniEx4.E1RA Scer\GAL4^pnr-MD237RA