Reference Report

Reference
Citation	Schnizler, K., Saeger, B., Pfeffer, C., Gerbaulet, A., Ebbinghaus-Kintscher, U., Methfessel, C., Franken, E.M., Raming, K., Wetzel, C.H., Saras, A., Pusch, H., Hatt, H., Gisselmann, G. (2005). A novel chloride channel in Drosophila melanogaster is inhibited by protons. J. Biol. Chem. 280(16): 16254--16262. (Export to RIS)
FlyBase ID	FBrf0187801
Publication Type	Research paper
PubMed ID	15713676
PubMed Abstract	A systematic analysis of the Drosophila genome data reveals the existence of pHCl, a novel member of ligand-gated ion channel subunits. pHCl shows nearly identical similarity to glutamate-, glycine-, and histamine-gated ion channels, does however not belong to any of these ion channel types. We identified three different sites, where splicing generates multiple transcripts of the pHCl mRNA. The pHCl is expressed in Drosophila embryo, larvae, pupae, and the adult fly. In embryos, in situ hybridization detected pHCl in the neural cord and the hindgut. Functional expression of the three different splice variants of pHCl in oocytes of Xenopus laevis and Sf9 cells induces a chloride current with a linear current-voltage relationship that is inhibited by extracellular protons and activated by avermectins in a pH-dependent manner. Further, currents through pHCl channels were induced by a raise in temperature. Our data give genetic and electrophysiological evidence that pHCl is a member of a new branch of ligand-gated ion channels in invertebrates with, however, a hitherto unique combination of pharmacological and biophysical properties.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Biol. Chem.
Title	Journal of Biological Chemistry
Publication Year	1905-
ISBN/ISSN	0021-9258
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Genes (1)
	pHCl