Reference Report

Reference
Citation	Beronja, S., Laprise, P., Papoulas, O., Pellikka, M., Sisson, J., Tepass, U. (2005). Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells. J. Cell Biol. 169(4): 635--646. (Export to RIS)
FlyBase ID	FBrf0187815
Publication Type	Research paper
PubMed ID	15897260
PubMed Abstract	Polarized exocytosis plays a major role in development and cell differentiation but the mechanisms that target exocytosis to specific membrane domains in animal cells are still poorly understood. We characterized Drosophila Sec6, a component of the exocyst complex that is believed to tether secretory vesicles to specific plasma membrane sites. sec6 mutations cause cell lethality and disrupt plasma membrane growth. In developing photoreceptor cells (PRCs), Sec6 but not Sec5 or Sec8 shows accumulation at adherens junctions. In late PRCs, Sec6, Sec5, and Sec8 colocalize at the rhabdomere, the light sensing subdomain of the apical membrane. PRCs with reduced Sec6 function accumulate secretory vesicles and fail to transport proteins to the rhabdomere, but show normal localization of proteins to the apical stalk membrane and the basolateral membrane. Furthermore, we show that Rab11 forms a complex with Sec5 and that Sec5 interacts with Sec6 suggesting that the exocyst is a Rab11 effector that facilitates protein transport to the apical rhabdomere in Drosophila PRCs.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (2)
	Df(2R)PC4 Df(2R)sec6¹⁷⁵
Alleles (11)
	arm³ Eip55E^EP2021 Rab11^{Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^da.G32 Scer\GAL4^ey.PH Scer\GAL4^ninaE.PHS sec6²⁰ sec6¹²⁵ sec6¹⁷⁵ sec6^{Scer\UAS.P\T.cBa} shi²
Constructs (5)
	P{GAL4-da.G32} P{GAL4-ey.H} P{Rh1-GAL4.HS} P{UASp-sec6.B} P{UAS-Rab11-GFP}
Genes (17)
	Act5C Act42A arm chp crb Eip55E futsch ninaE Patj Rab11 Scer\GAL4 sec5 sec6 sec8 shg shi α-Cat
Insertions (1)
	P{EP}Eip55E^EP2021
Natural transposons (1)
	P-element