Reference Report

Reference
Citation	Pulipparacharuvil, S., Akbar, M.A., Ray, S., Sevrioukov, E.A., Haberman, A.S., Rohrer, J., Kramer, H. (2005). Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules. J. Cell Sci. 118(16): 3663--3673. (Export to RIS)
FlyBase ID	FBrf0187837
Publication Type	Research paper
PubMed ID	16046475
PubMed Abstract	Mutations that disrupt trafficking to lysosomes and lysosome-related organelles cause multiple diseases, including Hermansky-Pudlak syndrome. The Drosophila eye is a model system for analyzing such mutations. The eye-color genes carnation and deep orange encode two subunits of the Vps-C protein complex required for endosomal trafficking and pigment-granule biogenesis. Here we demonstrate that dVps16A (CG8454) encodes another Vps-C subunit. Biochemical experiments revealed a specific interaction between the dVps16A C-terminus and the Sec1/Munc18 homolog Carnation but not its closest homolog, dVps33B. Instead, dVps33B interacted with a related protein, dVps16B (CG18112). Deep orange bound both Vps16 homologs. Like a deep orange null mutation, eye-specific RNAi-induced knockdown of dVps16A inhibited lysosomal delivery of internalized ligands and interfered with biogenesis of pigment granules. Ubiquitous knockdown of dVps16A was lethal. Together, these findings demonstrate that Drosophila Vps16A is essential for lysosomal trafficking. Furthermore, metazoans have two types of Vps-C complexes with non-redundant functions.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Alleles (11)
	car¹ dor¹ dor⁴ dor⁸ Hsap\LAMP1^{Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^da.G32 Scer\GAL4^ey.PH Scer\GAL4^GMR.PF T:Avic\GFP^EGFP Vps16A^{dsRNA.Scer\UAS.WIZ} w^+mC
Constructs (4)
	P{GAL4-da.G32} P{GAL4-ey.H} P{UAS-GFP-LAMP} P{UAS-Vps16A.WIZ}
Genes (14)
	boss car dor Dpse\GA14802 hk Hsap\LAMP1 Rop sca Scer\GAL4 Syx7 Vps16A Vps16B Vps33B w
Natural transposons (1)
	P-element