Reference Report

Reference
Citation	Andersen, R., Li, Y., Resseguie, M., Brenman, J.E. (2005). Calcium/calmodulin-dependent protein kinase II alters structural plasticity and cytoskeletal dynamics in Drosophila. J. Neurosci. 25(39): 8878--8888. (Export to RIS)
FlyBase ID	FBrf0187896
Publication Type	Research paper
PubMed ID	16192377
PubMed Abstract	Drosophila dendritic arborization (da) neurons contain subclasses of neurons with distinct dendritic morphologies. We investigated calcium/calmodulin-dependent protein kinase II (CaMKII) regulation of dendritic structure and dynamics in vivo using optically transparent Drosophila larvae. CaMKII increases the dynamic nature and formation of dendritic filopodia throughout larval development but only affects neurons that normally contain dendritic filopodia. In parallel, we examined the effects of Rac1 activity on dendritic structure to explore signaling specificity. In contrast to CaMKII activity, Rac1 does not alter filopodia stability but instead causes de novo filopodia formation on all da neurons. Although both mediators increase cytoskeletal turnover, measured by fluorescence recovery after photobleaching experiments, only CaMKII increases the dynamic nature of dendritic filopodia. CaMKII signaling thus appears to use mechanisms and machinery distinct from Rac1 signaling. This study illustrates a molecular means of uncoupling cytoskeletal regulation from morphological regulation. Our results suggest that Drosophila dendritic filopodia may share some cytoskeletal regulatory mechanisms with mammalian dendritic filopodia. Furthermore, general dendrite cytoskeletal compartmentalization is conserved in multipolar neurons.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Alleles (15)
	Act5C^{Scer\UAS.T:Avic\GFP} Avic\GFP^Scer\UAS.cUa Avic\GFP^{Scer\UAS.T:Btau\MAPT} CaMKII^Scer\UAS.cKa CaMKII^{T287A.Scer\UAS} CaMKII^{T287D.Scer\UAS} Disc\RFP^{Scer\UAS.T:Myr-Src64B} Khc::nod^{Scer\UAS.P\T.T:Ecol\lacZ} Khc::nod^{Scer\UAS.T:Avic\GFP-EGFP} Khc::nod^{Scer\UAS.T:Ecol\lacZ} Moe^{GMA.Scer\UAS.T:Avic\GFP-S65T} Rac1^Scer\UAS.cLa Scer\GAL4^109(2)80 T:Avic\GFP^EGFP T:Avic\GFP^S65T
Constructs (2)
	P{UAS-GMA} P{UAS-nod.GFP}
Genes (12)
	Act5C Avic\GFP CaMKII Disc\RFP futsch Khc::nod Moe nod Rac1 Scer\GAL4 T:Avic\GFP T:Myr-Src64B