Reference Report

Reference
Citation	Reiff, D.F., Ihring, A., Guerrero, G., Isacoff, E.Y., Joesch, M., Nakai, J., Borst, A. (2005). In vivo performance of genetically encoded indicators of neural activity in flies. J. Neurosci. 25(19): 4766--4778. (Export to RIS)
FlyBase ID	FBrf0187903
Publication Type	Research paper
PubMed ID	15888652
PubMed Abstract	Genetically encoded fluorescent probes of neural activity represent new promising tools for systems neuroscience. Here, we present a comparative in vivo analysis of 10 different genetically encoded calcium indicators, as well as the pH-sensitive synapto-pHluorin. We analyzed their fluorescence changes in presynaptic boutons of the Drosophila larval neuromuscular junction. Robust neural activity did not result in any or noteworthy fluorescence changes when Flash-Pericam, Camgaroo-1, and Camgaroo-2 were expressed. However, calculated on the raw data, fractional fluorescence changes up to 18% were reported by synapto-pHluorin, Yellow Cameleon 2.0, 2.3, and 3.3, Inverse-Pericam, GCaMP1.3, GCaMP1.6, and the troponin C-based calcium sensor TN-L15. The response characteristics of all of these indicators differed considerably from each other, with GCaMP1.6 reporting high rates of neural activity with the largest and fastest fluorescence changes. However, GCaMP1.6 suffered from photobleaching, whereas the fluorescence signals of the double-chromophore indicators were in general smaller but more photostable and reproducible, with TN-L15 showing the fastest rise of the signals at lower activity rates. We show for GCaMP1.3 and YC3.3 that an expanded range of neural activity evoked fairly linear fluorescence changes and a corresponding linear increase in the signal-to-noise ratio (SNR). The expression level of the indicator biased the signal kinetics and SNR, whereas the signal amplitude was independent. The presented data will be useful for in vivo experiments with respect to the selection of an appropriate indicator, as well as for the correct interpretation of the optical signals.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Alleles (16)
	Avic\GFP^{Cameleon2.0.Scer\UAS} Avic\GFP^{Cameleon2.3.Scer\UAS} Avic\GFP^{Cameleon3.3.Scer\UAS} Avic\GFP^{camgaroo1.Scer\UAS.cRa} Avic\GFP^{camgaroo2.Scer\UAS.cRa} Avic\GFP^{FlashPericam.Scer\UAS} Avic\GFP^{GCaMP1.3.Scer\UAS.cRa} Avic\GFP^{GCaMP1.3.Scer\UAS.cZa} Avic\GFP^{GCaMP1.6.Scer\UAS} Avic\GFP^{InversePericam.Scer\UAS} Avic\GFP^{pHluorinSE.Scer\UAS.T:Mmus\Vamp2} Avic\GFP^{TN-L15.Scer\UAS.T:Ggal\TNNC2} Scer\GAL4^elav-C155 T:Avic\GFP^cpEGFP T:Avic\GFP^ECFP T:Avic\GFP^EYFP
Constructs (12)
	P{UAS-Cameleon.2.0} P{UAS-Cameleon.2.3} P{UAS-Cameleon.3.3} P{UAS-camgaroo-1.R} P{UAS-camgaroo-2.R} P{UAS-Flash-Pericam} P{UAS-GCaMP1.3.R} P{UAS-GCaMP1.3.Z} P{UAS-G-CaMP.1.6} P{UAS-Inverse-Pericam} P{UAS-synapto-pHluorinSE.Vamp2} P{UAS-TN-L15}
Genes (8)
	Avic\GFP Scer\GAL4 T:Ggal\MLCK T:Ggal\TNNC2 T:Mmus\Vamp2 T:Ocun\Mlck2 T:Rat\Cam T:Xlae\Cam
Insertions (1)
	P{GawB}elav^C155
Natural transposons (1)
	P-element