Reference Report

Reference
Citation	Wang, T., Montell, C. (2005). Rhodopsin formation in Drosophila is dependent on the PINTA retinoid-binding protein. J. Neurosci. 25(21): 5187--5194. (Export to RIS)
FlyBase ID	FBrf0187905
Publication Type	Research paper
PubMed ID	15917458
PubMed Abstract	Retinoids participate in many essential processes including the initial event in photoreception. 11-cis-retinal binds to opsin and undergoes a light-driven isomerization to all-trans-retinal. In mammals, the all-trans-retinal is converted to vitamin A (all-trans-retinol) and is transported to the retinal pigment epithelium (RPE), where along with dietary vitamin A, it is converted into 11-cis-retinal. Although this cycle has been studied extensively in mammals, many questions remain, including the specific roles of retinoid-binding proteins. Here, we establish the Drosophila visual system as a genetic model for characterizing retinoid-binding proteins. In a genetic screen for mutations that affect the biosynthesis of rhodopsin, we identified a novel CRAL-TRIO domain protein, prolonged depolarization afterpotential is not apparent (PINTA), which binds to all-trans-retinol. We demonstrate that PINTA functions subsequent to the production of vitamin A and is expressed and required in the retinal pigment cells. These results represent the first genetic evidence for a role for the retinal pigment cells in the visual response. Moreover, our data implicate Drosophila retinal pigment cells as functioning in the conversion of dietary all-trans-retinol to 11-cis-retinal and suggest that these cells are the closest invertebrate equivalent to the RPE.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Aberrations (4)
	Df(3R)23D1 Df(3R)93F^x2 Df(3R)e-GC3 Df(3R)hh
Alleles (16)
	CG34376^EY05255 EP3303^EP3303 gl^unspecified hpo^Scer\UAS.cUa l(3)rQ178^rQ178 ninaD² ninaE⁷ ninaE⁸ ninaE¹⁷ pinta¹ pinta^Scer\UAS.cWa Scer\GAL4^GMR.PF Scer\GAL4^LL54 Scer\GAL4^ninaE.PD so^unspecified w^+mC
Constructs (3)
	P{GAL4-ninaE.GMR} P{ninaE-GAL4.D} P{UAS-pinta.W}
Genes (14)
	CG34376 EP3303 gl hpo l(3)rQ178 ninaB ninaD ninaE norpA pinta Rh4 Scer\GAL4 so w
Insertions (5)
	P{EP}EP561 P{EP}EP3303^EP3303 P{EPgy2}CG34376^EY05255 P{GawB}LL54 P{PZ}l(3)rQ178^rQ178
Natural transposons (1)
	P-element