Reference Report

Reference
Citation	Martin, M., Ahern-Djamali, S.M., Hoffmann, F.M., Saxton, W.M. (2005). Abl Tyrosine Kinase and Its Substrate Ena/VASP Have Functional Interactions with Kinesin-1. Mol. Biol. Cell 16(9): 4225--4230. (Export to RIS)
FlyBase ID	FBrf0188046
Publication Type	Research paper
PubMed ID	15975902
PubMed Abstract	Relatively little is known about how microtubule motors are controlled or about how the functions of different cytoskeletal systems are integrated. A yeast two-hybrid screen for proteins that bind to Drosophila Enabled (Ena), an actin polymerization factor that is negatively regulated by Abl tyrosine kinase, identified kinesin heavy chain (Khc), a member of the kinesin-1 subfamily of microtubule motors. Coimmunoprecipitation from Drosophila cytosol confirmed a physical interaction between Khc and Ena. Kinesin-1 motors can carry organelles and other macromolecular cargoes from neuronal cell bodies toward terminals in fast-axonal-transport. Ena distribution in larval axons was not affected by mutations in the Khc gene, suggesting that Ena is not itself a fast transport cargo of Drosophila kinesin-1. Genetic interaction tests showed that in a background sensitized by reduced Khc gene dosage, a reduction in Abl gene dosage caused distal paralysis and axonal swellings. A concomitant reduction in ena dosage rescued those defects. These results suggest that Ena/VASP, when not inhibited by the Abl pathway, can bind Khc and reduce its transport activity in axons.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Aberrations (8)
	Df(2R)Jp6 Df(3L)81k19 Df(3L)st-b11 Df(3L)st-E36 Df(3L)st-f13 Df(3L)st-j7 Df(3L)tra In(3L)std11
Alleles (12)
	Abl¹ Abl⁴ Baldspot^unspecified Dhc64C^ek1 ena²¹⁰ ena^Scer\UAS.cCa Khc⁶ Khc¹⁶ Scer\GAL4^Act5C.PI Scer\GAL4^elav-C155 Scer\GAL4^hs.PB Scer\GAL4^{Scer\FRT.Act5C}
Constructs (4)
	P{Act5C-GAL4} P{AyGAL4} P{GAL4-Hsp70.PB} P{UAS-ena.C}
Genes (13)
	Abl Baldspot CG13032 chic Csp Dhc64C ena Gαf Khc lark Scer\GAL4 sta ζCOP
Insertions (1)
	P{GawB}elav^C155