Reference Report

Reference
Citation	Roegiers, F., Jan, L.Y., Jan, Y.N. (2005). Regulation of membrane localization of Sanpodo by lethal giant larvae and neuralized in asymmetrically dividing cells of Drosophila sensory organs. Mol. Biol. Cell 16(8): 3480--3487. (Export to RIS)
FlyBase ID	FBrf0188062
Publication Type	Research paper
PubMed ID	15901829
PubMed Abstract	In Drosophila, asymmetric division occurs during proliferation of neural precursors of the central and peripheral nervous system (PNS), where a membrane-associated protein, Numb, is asymmetrically localized during cell division and is segregated to one of the two daughter cells (the pIIb cell) after mitosis. numb has been shown genetically to function as an antagonist of Notch signaling and also as a negative regulator of the membrane localization of Sanpodo, a four-pass transmembrane protein required for Notch signaling during asymmetric cell division in the CNS. Previously, we identified lethal giant larvae (lgl) as a gene required for numb-mediated inhibition of Notch in the adult PNS. In this study we show that Sanpodo is expressed in asymmetrically dividing precursor cells of the PNS and that Sanpodo internalization in the pIIb cell is dependent cytoskeletally associated Lgl. Lgl specifically regulates internalization of Sanpodo, likely through endocytosis, but is not required for the endocytosis Delta, which is a required step in the Notch-mediated cell fate decision during asymmetric cell division. Conversely, the E3 ubiquitin ligase neuralized is required for both Delta endocytosis and the internalization of Sanpodo. This study identifies a hitherto unreported role for Lgl as a regulator of Sanpodo during asymmetric cell division in the adult PNS.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Alleles (11)
	AP-2α^ear5 aPKC^ΔN.Scer\UAS Avic\GFP^{Scer\UAS.T:Btau\MAPT} l(2)gl⁴ Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} neur¹¹ numb² pon^{LD.Scer\UAS.T:Avic\GFP} Scer\GAL4^{neur-GAL4-A101} shi¹ spdo^C55
Constructs (4)
	P{UAS-aPKC.ΔN} P{UAS-GFP.btau} P{UAS-mCD8::GFP.L} P{UAS-pon.GFP.LD}
Genes (17)
	AP-2α aPKC ase Avic\GFP ct Dl elav l(2)gl Mmus\Cd8a N neur numb pon Sb Scer\GAL4 shi spdo
Insertions (1)
	P{GawB}neur^GAL4-A101