Reference Report

Reference
Citation	Sano, Y., Akimaru, H., Okamura, T., Nagao, T., Okada, M., Ishii, S. (2005). Drosophila activating transcription factor-2 is involved in stress response via activation by p38, but not c-Jun NH2-terminal kinase. Mol. Biol. Cell 16(6): 2934--2946. (Export to RIS)
FlyBase ID	FBrf0188065
Publication Type	Research paper
PubMed ID	15788564
PubMed Abstract	Activating transcription factor (ATF)-2 is a member of the ATF/cAMP response element-binding protein family of transcription factors, and its trans-activating capacity is enhanced by stress-activated protein kinases such as c-Jun NH(2)-terminal kinase (JNK) and p38. However, little is known about the in vivo roles played by ATF-2. Here, we identified the Drosophila homologue of ATF-2 (dATF-2) consisting of 381 amino acids. In response to UV irradiation and osmotic stress, Drosophila p38 (dp38), but not JNK, phosphorylates dATF-2 and enhances dATF-2-dependent transcription. Consistent with this, injection of dATF-2 double-stranded RNA (dsRNA) into embryos did not induce the dorsal closure defects that are commonly observed in the Drosophila JNK mutant. Furthermore, expression of the dominant-negative dp38 enhanced the aberrant wing phenotype caused by expression of a dominant-negative dATF-2. Similar genetic interactions between dATF-2 and the dMEKK1-dp38 signaling pathway also were observed in the osmotic stress-induced lethality of embryos. Loss of dATF-2 in Drosophila S2 cells by using dsRNA abrogated the induction of 40% of the osmotic stress-induced genes, including multiple immune response-related genes. This indicates that dATF-2 is a major transcriptional factor in stress-induced transcription. Thus, dATF-2 is critical for the p38-mediated stress response.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Alleles (24)
	Atf-2^{1-150.CMV.T:Scer\GAL4} Atf-2^{1-150.T59.61A.CMV.T:Scer\GAL4} Atf-2^{1-274.CMV.T:Scer\GAL4} Atf-2^{151-274.T:Scer\GAL4} Atf-2^{151-381.T:Scer\GAL4} Atf-2^{230-381.T:Scer\GAL4} Atf-2^{Act5C.T:Zzzz\FLAG} Atf-2^{bZIP.Scer\UAS} Atf-2^{CMV.T:Scer\GAL4} Atf-2^{L34P.CMV.T:Scer\GAL4} Atf-2^{T59.61A.CMV.T:Scer\GAL4} Atf-2^{T59A.Act5C.T:Zzzz\FLAG} Atf-2^{T61A.Act5C.T:Zzzz\FLAG} Atf-2^{ΔBR.CMV.T:Scer\GAL4} bsk¹ bsk^{Act5C.T:Ivir\HA1} lic^Act5C.PS Mekk1^Scer\UAS.cIa p38b^{Act5C.T:Ivir\HA1} p38b^DN.Scer\UAS Ppyr\LUC^4CRE.Adh Ppyr\LUC^{5x.Scer\UAS.Hsim\TK} Scer\GAL4^Bx-MS1096 Scer\GAL4^hs.PB
Constructs (4)
	P{GAL4-Hsp70.PB} P{UAS-Atf-2.bZIP} P{UAS-Mekk1.I} P{UAS-p38b.DN}
Genes (43)
	Atf-2 AttA AttC babos blot bsk CG3117 CG3505 CG4797 CG6231 CG8451 CG9733 CG10359 CG30088 CG30090 CG30103 CG34376 CG42235 Cpr97Eb dpp Dpt Drs Ela ex hep Jra lic Mekk1 mp Mpk2 mthl2 NT5E-2 Or71a p38b PGRP-SB1 PGRP-SC2 Ppyr\LUC Pu Scer\GAL4 spirit T:Ivir\HA1 T:Scer\GAL4 T:Zzzz\FLAG
Insertions (1)
	P{GawB}Bx^MS1096
Natural transposons (1)
	P-element