Reference Report

Reference
Citation	Sevrioukov, E.A., Moghrabi, N., Kuhn, M., Kramer, H. (2005). A mutation in dVps28 reveals a link between a subunit of the endosomal sorting complex required for transport-I complex and the actin cytoskeleton in Drosophila. Mol. Biol. Cell 16(5): 2301--2312. (Export to RIS)
FlyBase ID	FBrf0188067
Publication Type	Research paper
PubMed ID	15728719
PubMed Abstract	Proteins that constitute the endosomal sorting complex required for transport (ESCRT) are necessary for the sorting of proteins into multivesicular bodies (MVBs) and the budding of several enveloped viruses, including HIV-1. The first of these complexes, ESCRT-I, consists of three proteins: Vps28p, Vps37p, and Vps23p or Tsg101 in mammals. Here, we characterize a mutation in the Drosophila homolog of vps28. The dVps28 gene is essential: homozygous mutants die at the transition from the first to second instar. Removal of maternally contributed dVps28 causes early embryonic lethality. In such embryos lacking dVps28, several processes that require the actin cytoskeleton are perturbed, including axial migration of nuclei, formation of transient furrows during cortical divisions in syncytial embryos, and the subsequent cellularization. Defects in actin cytoskeleton organization also become apparent during sperm individualization in dVps28 mutant testis. Because dVps28 mutant cells contained MVBs, these defects are unlikely to be a secondary consequence of disrupted MVB formation and suggest an interaction between the actin cytoskeleton and endosomal membranes in Drosophila embryos earlier than previously appreciated.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Aberrations (1)
	Df(2R)CA53
Alleles (9)
	dor⁸ hk¹¹ hk^{MtnA.T:Ivir\HA1} or^49h Scer\GAL4^arm.PS TSG101^{MtnA.T:Ivir\HA1} Vps28^k16503 Vps28^{MtnA.T:Hsap\MYC} Vps28^Scer\UAS.cSa
Constructs (2)
	P{GAL4-arm.S} P{UAS-Vps28.S}
Genes (14)
	boss dah Dl dor hk nuf or Rab11 rl Scer\GAL4 T:Hsap\MYC T:Ivir\HA1 TSG101 Vps28
Insertions (1)
	P{lacW}Vps28^k16503
Natural transposons (1)
	P-element