Reference Report

Reference
Citation	Carre, C., Szymczak, D., Pidoux, J., Antoniewski, C. (2005). The histone H3 acetylase dGcn5 is a key player in Drosophila melanogaster metamorphosis. Mol. Cell. Biol. 25(18): 8228--8238. (Export to RIS)
FlyBase ID	FBrf0188098
Publication Type	Research paper
PubMed ID	16135811
PubMed Abstract	Although it has been well established that histone acetyltransferases (HATs) are involved in the modulation of chromatin structure and gene transcription, there is only little information on their developmental role in higher organisms. Gcn5 was the first transcription factor with HAT activity identified in eukaryotes. Here we report the isolation and characterization of Drosophila melanogaster dGcn5 mutants. Null dGcn5 alleles block the onset of both oogenesis and metamorphosis, while hypomorphic dGcn5 alleles impair the formation of adult appendages and cuticle. Strikingly, the dramatic loss of acetylation of the K9 and K14 lysine residues of histone H3 in dGcn5 mutants has no noticeable effect on larval tissues. In contrast, strong cell proliferation defects in imaginal tissues are observed. In vivo complementation experiments revealed that dGcn5 integrates specific functions in addition to chromosome binding and acetylation. Surprisingly, a dGcn5 variant protein with a deletion of the bromodomain, which has been shown to recognize acetylated histones, appears to be fully functional. Our results establish dGcn5 as a major histone H3 acetylase in Drosophila which plays a key role in the control of specific morphogenetic cascades during developmental transitions.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Aberrations (2)
	Df(3L)iro-2 Df(3L)sex204
Alleles (22)
	Gcn5^+t4.7 Gcn5^C137T Gcn5^{dsRNA.Scer\UAS} Gcn5^E333st Gcn5^Q186st Gcn5^Scer\UAS.cCa Gcn5^{ΔAda.Scer\UAS} Gcn5^{ΔBromo.Scer\UAS} Gcn5^{ΔHAT.Scer\UAS} Gcn5^{ΔPcaf.Scer\UAS} Gcn5^ΔT280-F285 Hsap\HIST2H2BE^{Scer\UAS.T:Avic\GFP-YFP} l(3)S147912^S147912 Scer\GAL4^da.G32 Scer\GAL4^drl-P15 Scer\GAL4^esg-NP5153 Scer\GAL4^vg.boundary sti^sex204 T:Avic\GFP^YFP tral^sex204 ver^S147910 ver^t4.7
Constructs (10)
	P{GAL4-da.G32} P{PL} P{UAS-H2B-T:Avic\GFP-YFP} P{UAS-Pcaf.C} P{UAS-Pcaf.dsRNA} P{UAS-Pcaf.ΔAda} P{UAS-Pcaf.ΔBromo} P{UAS-Pcaf.ΔHAT} P{UAS-Pcaf.ΔPcaf} P{vg-GAL4.boundary}
Genes (9)
	Gcn5 His3 His4 Hsap\HIST2H2BE Scer\GAL4 sti Su(var)205 tral ver
Insertions (3)
	P{GAL4}drl^P15 P{GawB}NTPase^NP5153 P{lacW}ver^S147910
Natural transposons (1)
	P-element