Reference Report

Reference
Citation	Rasse, T.M., Fouquet, W., Schmid, A., Kittel, R.J., Mertel, S., Sigrist, C.B., Schmidt, M., Guzman, A., Merino, C., Qin, G., Quentin, C., Madeo, F.F., Heckmann, M., Sigrist, S.J. (2005). Glutamate receptor dynamics organizing synapse formation in vivo. Nat. Neurosci. 8(7): 898--905. (Export to RIS)
FlyBase ID	FBrf0188163
Publication Type	Research paper
PubMed ID	16136672
PubMed Abstract	Insight into how glutamatergic synapses form in vivo is important for understanding developmental and experience-triggered changes of excitatory circuits. Here, we imaged postsynaptic densities (PSDs) expressing a functional, GFP-tagged glutamate receptor subunit (GluR-IIA(GFP)) at neuromuscular junctions of Drosophila melanogaster larvae for several days in vivo. New PSDs, associated with functional and structural presynaptic markers, formed independently of existing synapses and grew continuously until reaching a stable size within hours. Both in vivo photoactivation and photobleaching experiments showed that extrasynaptic receptors derived from diffuse, cell-wide pools preferentially entered growing PSDs. After entering PSDs, receptors were largely immobilized. In comparison, other postsynaptic proteins tested (PSD-95, NCAM and PAK homologs) exchanged faster and with no apparent preference for growing synapses. We show here that new glutamatergic synapses form de novo and not by partitioning processes from existing synapses, suggesting that the site-specific entry of particular glutamate receptor complexes directly controls the assembly of individual PSDs.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Neurosci.
Title	Nature Neuroscience
Publication Year	1998-
ISBN/ISSN	1097-6256
Data from Reference
Aberrations (2)
	Df(2L)cl-h4 Df(2L)SP22
Alleles (15)
	cac^{Scer\UAS.T:Avic\GFP-EGFP} dlg1^{S97N.Scer\UAS.T:Avic\GFP-EGFP} Fas2^Mue397 GluRIIA^+tRa GluRIIA^{T:Avic\GFP-EGFP} GluRIIA^{T:Avic\GFP-PA} GluRIIA^{T:Disc\RFP-mRFP} Pak^{Scer\UAS.T:Avic\GFP-EGFP} Pak^{Scer\UAS.T:Hsap\MYC} Scer\GAL4^C57 Scer\GAL4^G14 Sh^{Mhc.T:Avic\GFP-S65T,T:Hsap\CD8A} T:Avic\GFP^EGFP T:Avic\GFP^PA T:Disc\RFP^mRFP
Constructs (8)
	P{GluRIIA.GFP} P{GluRIIA.GFP-pa} P{GluRIIA^+tRa} P{GluRIIA-mRFP} P{Mhc.CD8-GFP-Sh} P{UAS-cac1-EGFP} P{UAS-dlg1.S97N-EGFP} P{UAS-Pak.GFP}
Genes (10)
	brp cac dlg1 Fas2 GluRIIA GluRIIB GluRIIC Pak Scer\GAL4 Sh
Insertions (3)
	P{GAL4}G14 P{GawB}C57 P{PTT-un1}Fas2^Mue397