Reference Report

Reference
Citation	Verstreken, P., Ly, C.V., Venken, K.J., Koh, T.W., Zhou, Y., Bellen, H.J. (2005). Synaptic mitochondria are critical for mobilization of reserve pool vesicles at Drosophila neuromuscular junctions. Neuron 47(3): 365--378. (Export to RIS)
FlyBase ID	FBrf0188182
Publication Type	Research paper
PubMed ID	16055061
PubMed Abstract	In a forward screen for genes affecting neurotransmission in Drosophila, we identified mutations in dynamin-related protein (drp1). DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses. Although resting Ca2+ is elevated at drp1 NMJs, basal synaptic properties are barely affected. However, during intense stimulation, mutants fail to maintain normal neurotransmission. Surprisingly, FM1-43 labeling indicates normal exo- and endocytosis, but a specific inability to mobilize reserve pool vesicles, which is partially rescued by exogenous ATP. Using a variety of drugs, we provide evidence that reserve pool recruitment depends on mitochondrial ATP production downstream of PKA signaling and that mitochondrial ATP limits myosin-propelled mobilization of reserve pool vesicles. Our data suggest a specific role for mitochondria in regulating synaptic strength.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Aberrations (1)
	Df(2L)Exel6008
Alleles (10)
	Avic\GFP^{S65T.Scer\UAS.T:Ivir\HA1,T:Hsap\mito} Drp1¹ Drp1² Drp1^+t9.4 Drp1^H7 Drp1^KG03815 Drp1^T26 Drp1^{T:Zzzz\FLAG,T:Zzzz\TC,T:Ivir\HA1} Scer\GAL4^D42 shi¹
Constructs (3)
	P{Drp1^+t9.4} P{FLAG-FlAsH-HA-Drp1} P{UAS-mito-HA-GFP.AP}
Genes (9)
	Avic\GFP dlg1 Drp1 futsch Scer\GAL4 shi T:Ivir\HA1 T:Zzzz\FLAG T:Zzzz\TC
Insertions (2)
	P{GawB}D42 P{SUPor-P}Drp1^KG03815
Natural transposons (1)
	P-element