Reference Report

Reference
Citation	Yano, H., Yamamoto-Hino, M., Abe, M., Kuwahara, R., Haraguchi, S., Kusaka, I., Awano, W., Kinoshita-Toyoda, A., Toyoda, H., Goto, S. (2005). Distinct functional units of the Golgi complex in Drosophila cells. Proc. Natl. Acad. Sci. U.S.A. 102(38): 13467--13472. (Export to RIS)
FlyBase ID	FBrf0188285
Publication Type	Research paper
PubMed ID	16174741
PubMed Abstract	A striking variety of glycosylation occur in the Golgi complex in a protein-specific manner, but how this diversity and specificity are achieved remains unclear. Here we show that stacked fragments (units) of the Golgi complex dispersed in Drosophila imaginal disk cells are functionally diverse. The UDP-sugar transporter FRINGE-CONNECTION (FRC) is localized to a subset of the Golgi units distinct from those harboring SULFATELESS (SFL), which modifies glucosaminoglycans (GAGs), and from those harboring the protease RHOMBOID (RHO), which processes the glycoprotein SPITZ (SPI). Whereas the glycosylation and function of NOTCH are affected in imaginal disks of frc mutants, those of SPI and of GAG core proteins are not, even though FRC transports a broad range of glycosylation substrates, suggesting that Golgi units containing FRC and those containing SFL or RHO are functionally separable. Distinct Golgi units containing FRC and RHO in embryos could also be separated biochemically by immunoisolation techniques. We also show that Tn-antigen glycan is localized only in a subset of the Golgi units distributed basally in a polarized cell. We propose that the different localizations among distinct Golgi units of molecules involved in glycosylation underlie the diversity of glycan modification.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (13)
	Avic\GFP^unspecified Ecol\lacZ^Dll.Scer\UAS Ecol\lacZ^unspecified fng^{Scer\UAS.cYa.T:Ivir\HA1} frc^R29 frc^RY34 frc^{Scer\UAS.T:Hsap\MYC} rho^{Scer\UAS.T:Ivir\HA1} rho^ve-1 S^Scer\UAS.cGa Scer\GAL4^arm.PS spi^{Scer\UAS.T:Hsap\MYC} ttv⁵²⁴
Constructs (7)
	P{Dll-UAS-lacZ} P{GAL4-arm.S} P{UAS-fng-HA.Y} P{UAS-frc.myc} P{UAS-myc-spi.L} P{UAS-rho.HA} P{UAS-Star}
Genes (23)
	Avic\GFP B Dll Ecol\lacZ elav fng frc futsch Glg1 GM130 N ptc rho S Scer\GAL4 sfl spi Syx16 T:Hsap\MYC T:Ivir\HA1 Tn ttv wg
Natural transposons (1)
	P-element