Open Close
Reference
Citation
DasGupta, R., Kaykas, A., Moon, R.T., Perrimon, N. (2005). Functional genomic analysis of the Wnt-wingless signaling pathway.  Science 308(5723): 826--833.
FlyBase ID
FBrf0188318
Publication Type
Research paper
Abstract

The Wnt-Wingless (Wg) pathway is one of a core set of evolutionarily conserved signaling pathways that regulates many aspects of metazoan development. Aberrant Wnt signaling has been linked to human disease. In the present study, we used a genomewide RNA interference (RNAi) screen in Drosophila cells to screen for regulators of the Wnt pathway. We identified 238 potential regulators, which include known pathway components, genes with functions not previously linked to this pathway, and genes with no previously assigned functions. Reciprocal-Best-Blast analyses reveal that 50% of the genes identified in the screen have human orthologs, of which approximately 18% are associated with human disease. Functional assays of selected genes from the cell-based screen in Drosophila, mammalian cells, and zebrafish embryos demonstrated that these genes have evolutionarily conserved functions in Wnt signaling. High-throughput RNAi screens in cultured cells, followed by functional analyses in model organisms, prove to be a rapid means of identifying regulators of signaling pathways implicated in development and disease.

PubMed ID
PubMed Central ID
Related Publication(s)
Review

A functional genomics approach to identify new regulators of Wnt signaling.
Furlong, 2005, Dev. Cell 8(5): 624--626 [FBrf0187406]

Note

Cell biology. Wnt signaling glows with RNAi.
Fearon and Cadigan, 2005, Science 308(5723): 801--803 [FBrf0188320]

Screening for Wnt components.
Niemitz, 2005, Nat. Genet. 37(5): 464 [FBrf0188727]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Science
    Title
    Science
    Publication Year
    1895-
    ISBN/ISSN
    0036-8075
    Data From Reference
    Alleles (2)
    Genes (29)
    Cell Lines (1)
    Insertions (1)
    Transgenic Constructs (1)