Reference Report

Reference
Citation	Bruey-Sedano, N., Alabouvette, J., Lestradet, M., Hong, L., Girard, A., Gervasio, E., Quennedey, B., Charles, J.P. (2005). The Drosophila ACP65A cuticle gene: deletion scanning analysis of cis-regulatory sequences and regulation by DHR38. genesis 43(1): 17--27. (Export to RIS)
FlyBase ID	FBrf0188378
Publication Type	Research paper
PubMed ID	16106360
PubMed Abstract	The regulatory sequences of the Drosophila ACP65A cuticle gene were analyzed in vivo in transgenic flies, using both fusion genes constructs and transposase-mediated deletions within a P element containing ACP65A regulatory sequences fused to the lacZ gene (deletion scanning). The sequences located between -594 and +161 are sufficient to confer both temporal and spatial expression specificities, indicating the presence of tissue-specific enhancers and response elements to hormone-induced factors. In addition, timing of expression and tissue-specificity appear to be controlled by distinct cis-regulatory elements, which suggests the existence of independent hormonal and tissue-specific signaling pathways. Gain and loss of function studies also implicate DHR38, the Drosophila homolog of the vertebrate NGFI-B-type nuclear receptors, as an important activator of the ACP65A gene.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	genesis
Title	genesis
Publication Year	2000-
ISBN/ISSN	1526-954X 1526-968X
Data from Reference
Alleles (22)
	Avic\GFP^{S65T.Acp65Aa.256} Avic\GFP^{S65T.Acp65Aa.744} Avic\GFP^{S65T.Acp65Aa.1768} Avic\GFP^{S65T.Acp65Aa.2529} Ecol\lacZ^Acp65Aa.143 Ecol\lacZ^Acp65Aa.172 Ecol\lacZ^Acp65Aa.191 Ecol\lacZ^Acp65Aa.196 Ecol\lacZ^{Acp65Aa.213-5'intr} Ecol\lacZ^Acp65Aa.213 Ecol\lacZ^Acp65Aa.237 Ecol\lacZ^Acp65Aa.594 Ecol\lacZ^Acp65Aa.779 Ecol\lacZ^Acp65Aa.865 Ecol\lacZ^Acp65Aa.963 Ecol\lacZ^Acp65Aa.1807 Ecol\lacZ^Acp65Aa.2529 Hr38⁰²³⁰⁶ Hr38^hs.11 Hr38^hs.61 usp^hs.PO w^+mC
Constructs (20)
	P{Acp65Aa-GFP.256} P{Acp65Aa-GFP.744} P{Acp65Aa-GFP.1768} P{Acp65Aa-GFP.2529} P{Acp65Aa-lacZ.143} P{Acp65Aa-lacZ.172} P{Acp65Aa-lacZ.191} P{Acp65Aa-lacZ.196} P{Acp65Aa-lacZ.213-5'intr} P{Acp65Aa-lacZ.213} P{Acp65Aa-lacZ.237} P{Acp65Aa-lacZ.594} P{Acp65Aa-lacZ.779} P{Acp65Aa-lacZ.865} P{Acp65Aa-lacZ.963} P{Acp65Aa-lacZ.1807} P{Acp65Aa-lacZ.2529} P{hs-Hr38.11} P{hs-Hr38.61} P{hs-usp}
Genes (6)
	Acp65Aa Avic\GFP Ecol\lacZ Hr38 usp w
Natural transposons (1)
	P-element