Reference Report

Reference
Citation	Apidianakis, Y., Mindrinos, M.N., Xiao, W., Lau, G.W., Baldini, R.L., Davis, R.W., Rahme, L.G. (2005). Profiling early infection responses: Pseudomonas aeruginosa eludes host defenses by suppressing antimicrobial peptide gene expression. Proc. Natl. Acad. Sci. U.S.A. 102(7): 2573--2578. (Export to RIS)
FlyBase ID	FBrf0188443
Publication Type	Research paper
PubMed ID	15695583
PubMed Abstract	Insights into the host factors and mechanisms mediating the primary host responses after pathogen presentation remain limited, due in part to the complexity and genetic intractability of host systems. Here, we employ the model Drosophila melanogaster to dissect and identify early host responses that function in the initiation and progression of Pseudomonas aeruginosa pathogenesis. First, we use immune potentiation and genetic studies to demonstrate that flies mount a heightened defense against the highly virulent P. aeruginosa strain PA14 when first inoculated with strain CF5, which is avirulent in flies; this effect is mediated via the Imd and Toll signaling pathways. Second, we use whole-genome expression profiling to assess and compare the Drosophila early defense responses triggered by the PA14 vs. CF5 strains to identify genes whose expression patterns are different in susceptible vs. resistant host-pathogen interactions, respectively. Our results identify pathogenesis- and defense-specific genes and uncover a previously undescribed mechanism used by P. aeruginosa in the initial stages of its host interaction: suppression of Drosophila defense responses by limiting antimicrobial peptide gene expression. These results provide insights into the genetic factors that mediate or restrict pathogenesis during the early stages of the bacterial-host interaction to advance our understanding of P. aeruginosa-human infections.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (11)
	AttA^Scer\UAS.cTa CecA1^Scer\UAS.cTa Def^Scer\UAS.cTa Dpt^Scer\UAS.cTa Dro^Scer\UAS.cTa Drs^Scer\UAS.cTa imd¹ Rel^E20 Scer\GAL4^da.G32 spz² spz⁴
Constructs (8)
	P{GAL4-da.G32} P{UAS-AttA.T} P{UAS-CecA1.T} P{UAS-Def.T} P{UAS-Dpt.T} P{UAS-Dro.T} P{UAS-Drs.T} P{UAS-Drs-dsRNA}
Genes (90)
	Abi Acp1 Acp65Aa Act79B Act88F Actn ACXA AttA AttB Ccp84Ab CecA1 CecA2 CecB CecC Cg25C CG1648 CG3699 CG4757 CG7298 CG8481 CG8736 CG9948 CG13579 CG17121 CG31324 CG42825 Cht7 ci Cpr47Ea Cpr49Ae Cpr62Bc Cpr92F Cyp6g1 Cyp12d1-d daw Def Dpt DptB Dro Drs fln Fst Gasp Gr21a GstS1 Hers Hsp22 Hsp26 Hsp68 Hsp70Bbb Hsp70Bc IM23 imd Jon65Aii Lsp2 LysB LysC LysD LysE Mhc MSBP Msp-300 mth mthl6 Mtk obst-A Peritrophin-15a PGRP-SB1 Prm Rbcn-3B Rel Rgk2 Sap-r Scer\GAL4 sesB Skp2 Sodh-1 spz Strn-Mlck Tep2 to TotM TpnC25D TpnC47D verm Vha68-1 VhaPPA1-1 wbl wupA αTry
Insertions (1)
	P{GAL4-da.G32}UH1