Reference Report

Reference
Citation	Sanyal, S., Consoulas, C., Kuromi, H., Basole, A., Mukai, L., Kidokoro, Y., Krishnan, K.S., Ramaswami, M. (2005). Analysis of conditional paralytic mutants in Drosophila sarco-endoplasmic reticulum calcium ATPase reveals novel mechanisms for regulating membrane excitability. Genetics 169(2): 737--750. (Export to RIS)
FlyBase ID	FBrf0188489
Publication Type	Research paper
PubMed ID	15520268
PubMed Abstract	Individual contributions made by different calcium release and sequestration mechanisms to various aspects of excitable cell physiology are incompletely understood. SERCA, a sarco-endoplasmic reticulum calcium ATPase, being the main agent for calcium uptake into the ER, plays a central role in this process. By isolation and extensive characterization of conditional mutations in the Drosophila SERCA gene, we describe novel roles of this key protein in neuromuscular physiology and enable a genetic analysis of SERCA function. At motor nerve terminals, SERCA inhibition retards calcium sequestration and reduces the amplitude of evoked excitatory junctional currents. This suggests a direct contribution of store-derived calcium in determining the quantal content of evoked release. Conditional paralysis of SERCA mutants is also marked by prolonged neural activity-driven muscle contraction, thus reflecting the phylogenetically conserved role of SERCA in terminating contraction. Further analysis of ionic currents from mutants uncovers SERCA-dependent mechanisms regulating voltage-gated calcium channels and calcium-activated potassium channels that together control muscle excitability. Finally, our identification of dominant loss-of-function mutations in SERCA indicates novel intra- and intermolecular interactions for SERCA in vivo, overlooked by current structural models.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (3)
	Df(2R)bw-S46 Df(2R)egl2 Df(2R)or-BR11
Alleles (17)
	Ca-P60A^{Kum170.Scer\UAS} Ca-P60A^Kum170 Ca-P60A^Kum295 Ca-P60A^KumP52 Ca-P60A^l1 Ca-P60A^unspecified para^ts1 Scer\GAL4^arm.PS Scer\GAL4^Cha.7.4 Scer\GAL4^elav-C155 Scer\GAL4^futsch-C380 Scer\GAL4^how-24B Scer\GAL4^Mef2.PR Scer\GAL4^Mhc.PW Scer\GAL4^shi.PS Scer\GAL4^αTub84B.PL w^+mC
Constructs (7)
	P{Cha-GAL4.7.4} P{GAL4-arm.S} P{GAL4-Mef2.R} P{GAL4-Mhc.W} P{shi-GAL4.S} P{tubP-GAL4} P{UAS-Ca-P60A.Kum170}
Genes (4)
	Ca-P60A para Scer\GAL4 w
Insertions (4)
	P{EP}Ca-P60A^KumP52 P{GAL4}C380 P{GawB}elav^C155 P{GawB}how^24B