Reference Report

Reference
Citation	Jones, W.M., Bejsovec, A. (2005). RacGap50C negatively regulates wingless pathway activity during Drosophila embryonic development. Genetics 169(4): 2075--2086.
FlyBase ID	FBrf0188508
Type of publication	Research paper
Offprint Available	No
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PubMed ID	15695356
PubMed Abstract	The Wingless (Wg)/Wnt signal transduction pathway directs a variety of cell fate decisions in developing animal embryos. Despite the identification of many Wg pathway components to date, it is still not clear how these elements work together to generate cellular identities. In the ventral epidermis of Drosophila embryos, Wg specifies cells to secrete a characteristic pattern of denticles and naked cuticle that decorate the larval cuticle at the end of embryonic development. We have used the Drosophila ventral epidermis as our assay system in a series of genetic screens to identify new components involved in Wg signaling. Two mutant lines that modify wg-mediated epidermal patterning represent the first loss-of-function mutations in the RacGap50C gene. These mutations on their own cause increased stabilization of Armadillo and cuticle pattern disruptions that include replacement of ventral denticles with naked cuticle, which suggests that the mutant embryos suffer from ectopic Wg pathway activation. In addition, RacGap50C mutations interact genetically with naked cuticle and Axin, known negative regulators of the Wg pathway. These phenotypes suggest that the RacGap50C gene product participates in the negative regulation of Wg pathway activity.
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Language of publication	English
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Abbreviation	Genetics
Title	Genetics
Authors
Volume range	1-
Year range	1916-
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Place of publication	Austin, TX, etc
Language of publication	English
ISBN/ISSN	0016-6731
CODEN	GENTAE
Data from Reference
Aberrations (2)
	Df(2R)CX1 Df(2R)vg-B
Alleles (16)
	Axn^{Scer\UAS.T:Avic\GFP} Rac1^N17.Scer\UAS Rac1^V12.Scer\UAS Scer\GAL4^arm.PS Scer\GAL4^e22c Scer\GAL4^prd.RG1 Scer\GAL4^wg.PM nkd² nkd^Scer\UAS.cZa tum^AR2 tum^DH15 tum^Scer\UAS.cSa tum^{ΔE1E.Scer\UAS} wg^PE2 wg^l-12 wg^l-17
Constructs (9)
	P{GAL4-arm.S} P{GAL4-prd.F} P{GAL4-wg.M} P{UAS-Axn.GFP} P{UAS-Rac1.N17} P{UAS-Rac1.V12} P{UAS-RacGAP.S} P{UAS-RacGAP.ΔE1E} P{UAS-nkd.Z}
Genes (11)
	Axn Rac1 Scer\GAL4 T:Avic\GFP arm c cn cora nkd tum wg
Insertions (3)
	P{EP}EP2423 P{en2.4-GAL4}e22c P{lacW}shot^k03010