Isolation and characterization of Df(3L)BSC119
Rachel Andrade and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC119 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f01070 and P{XP}d07033. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, w1118; PBac{WH}f01070/P{XP}d07033 females crossed to w1118; P{w+mC=hs-hid}2, wgSp-1/CyO males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC119 from the segment of PBac{WH}f01070 to the left of its FRT site and the segment of P{XP}d07033 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}f01070 to be at Release 3 genomic coordinate 2580837 on chromosome arm 3L and the insertion site of P{XP}d07033 to be at Release 3 genomic coordinate 2804237 on arm 3L. The Gene Disruption Project determined the insertion site of PBac{WH}f01070 to be at Release 3 genomic coordinate 2580912 on arm 3L and the insertion site of P{XP}d07033 to be at Release 3 genomic coordinate 2804243 on arm 3L. The cytological breakpoints of Df(3L)BSC119 predicted from these coordinates are 62E7;62F5. Polytene chromosome squashes verified the presence of a deletion. Df(3L)BSC119 failed to complement Df(3L)BSC23.