Isolation and characterization of Df(3L)BSC126
Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC126 was isolated as a FLP recombinase-induced recombination event involving P{XP}d04808 and PBac{WH}CG32343f06767. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118, P{hsFLP}1; P{XP}d04808/ PBac{WH}CG32343f06767 females crossed to w1118; wgSp-1/CyO; sensLy-1/TM6B, Tb1 males. The females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC126 from the segment of P{XP}d04808 to the left of its FRT site and the segment of and PBac{WH}CG32343f06767 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC126 predicted from the transposable element insertions sites are 61B3;61C1. It failed to complement Df(3L)Exel6084.