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Citation
Tanji, T., Ohashi-Kobayashi, A., Natori, S. (2006). Participation of a galactose-specific C-type lectin in Drosophila immunity.  Biochem. J. 396(1): 127--138.
FlyBase ID
FBrf0189817
Publication Type
Research paper
Abstract

A galactose-specific C-type lectin has been purified from a pupal extract of Drosophila melanogaster. This lectin gene, named DL1 (Drosophila lectin 1), is part of a gene cluster with the other two galactose-specific C-type lectin genes, named DL2 (Drosophila lectin 2) and DL3 (Drosophila lectin 3). These three genes are expressed differentially in fruit fly, but show similar haemagglutinating activities. The present study characterized the biochemical and biological properties of the DL1 protein. The recombinant DL1 protein bound to Escherichia coli and Erwinia chrysanthemi, but not to other Gram-negative or any other kinds of microbial strains that have been investigated. In addition, DL1 agglutinated E. coli and markedly intensified the association of a Drosophila haemocytes-derived cell line with E. coli. For in vivo genetic analysis of the lectin genes, we also established a null-mutant Drosophila. The induction of inducible antibacterial peptide genes was not impaired in the DL1 mutant, suggesting that the galactose-specific C-type lectin does not participate in the induction of antibacterial peptides, but possibly participates in the immune response via the haemocyte-mediated mechanism.

PubMed ID
PubMed Central ID
PMC1450005 (PMC) (EuropePMC)
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Secondary IDs
  • FBrf0192779
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
Biochem. J.
Title
The Biochemical Journal
Publication Year
1906-
ISBN/ISSN
0264-6021
Data From Reference
Aberrations (1)
Alleles (6)
Gene Groups (1)
Genes (45)
Natural transposons (1)
Insertions (2)
Transgenic Constructs (2)